NDLI: Viability and Isolation of Marine Bacteria by Dilution Culture: Theory, Procedures, and Initial Results

Please wait, while we are loading the content...

Year: 2016, Volume: 82

Year: 2015, Volume: 82

Year: 2015, Volume: 81

Year: 2014, Volume: 80

Year: 2013, Volume: 79

Year: 2012, Volume: 78

Year: 2011, Volume: 77

Year: 2010, Volume: 76

Year: 2009, Volume: 75

Year: 2008, Volume: 74

Year: 2007, Volume: 73

Year: 2006, Volume: 72

Year: 2005, Volume: 71

Year: 2004, Volume: 70

Year: 2003, Volume: 69

Year: 2002, Volume: 69

Year: 2002, Volume: 68

Year: 2001, Volume: 67

Year: 2000, Volume: 66

Year: 1999, Volume: 65

Year: 1998, Volume: 64

Year: 1997, Volume: 63

Year: 1996, Volume: 62

Year: 1995, Volume: 61

Year: 1994, Volume: 60

Year: 1993, Volume: 59

Year: 1993, Volume: 59, Issue: 12

Year: 1993, Volume: 59, Issue: 11

Year: 1993, Volume: 59, Issue: 10

Year: 1993, Volume: 59, Issue: 9

Year: 1993, Volume: 59, Issue: 8

Year: 1993, Volume: 59, Issue: 7

Year: 1993, Volume: 59, Issue: 6

Year: 1993, Volume: 59, Issue: 5

Year: 1993, Volume: 59, Issue: 4

Year: 1993, Volume: 59, Issue: 3

Amounts of viable anaerobes, methanogens, and bacterial fermentation products in feces of rats fed high-fiber or fiber-free diets.

In vivo labeling of Escherichia coli cell envelope proteins with N-hydroxysuccinimide esters of biotin.

Modification of the Fe Protein of Nitrogenase in Natural Populations of Trichodesmium thiebautii

A Method for Detection of Pseudobactin, the Siderophore Produced by a Plant-Growth-Promoting Pseudomonas Strain, in the Barley Rhizosphere

Monitoring the enrichment and isolation of sulfate-reducing bacteria by using oligonucleotide hybridization probes designed from environmentally derived 16S rRNA sequences.

Polymerase chain reaction amplification of naphthalene-catabolic and 16S rRNA gene sequences from indigenous sediment bacteria.

Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.

Transformation of 3- and 4-Picoline under Sulfate-Reducing Conditions

Purification and Properties of an S-Adenosylmethionine: 2,4-Disubstituted Phenol O-Methyltransferase from Phanerochaete chrysosporium

Abundance of Virus-Sized Non-DNase-Digestible DNA (Coated DNA) in Eutrophic Seawater

Distribution of viral abundance in the reef environment of Key Largo, Florida.

Microbiological transformations of lipids: acyl-specific hydrolysis of lard by yeasts.

Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae.

Dissimilatory Fe(III) Reduction by the Marine Microorganism Desulfuromonas acetoxidans

Metabolic Activity and Population Dynamics of Rhizobia Introduced into Unamended and Bentonite-Amended Loamy Sand

Glucose and carbon dioxide metabolism by Succinivibrio dextrinosolvens.

Degradation of benzene, toluene, ethylbenzene, and xylenes (BTEX) by the lignin-degrading basidiomycete Phanerochaete chrysosporium.

Regulation and Characterization of Xylanolytic Enzymes of Thermoanaerobacterium saccharolyticum B6A-RI

Antibody-magnetite method for selective concentration of Giardia lamblia cysts from water samples.

ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503: cloning and characterization of two ScrFI methylase genes.

Evaluation of colilert-marine water for detection of total coliforms and Escherichia coli in the marine environment.

Identification of metabolites from the degradation of fluoranthene by Mycobacterium sp. strain PYR-1.

Conjugal Gene Transfer to Aquatic Bacteria Detected by the Generation of a New Phenotype †

High-level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20-kilodalton protein, and the coexpressed gene products are synergistic in their toxicity to mosquitoes.

Aspartate aminotransferase and tylosin biosynthesis in Streptomyces fradiae.

Isolation and characterization of an extracellular glycosylated protein complex from Clostridium thermosaccharolyticum with pectin methylesterase and polygalacturonate hydrolase activity.

Involvement of an Intracellular Oligogalacturonate Hydrolase in Metabolism of Pectin by Clostridium thermosaccharolyticum

Plasmid Transfer between Marine Bacteria in the Aqueous Phase and Biofilms in Reactor Microcosms

Characterization of Xanthomonas campestris Pathovars by rRNA Gene Restriction Patterns

Selection of Pseudomonas sp. strain HBP1 Prp for metabolism of 2-propylphenol and elucidation of the degradative pathway.

Enhanced Carotenoid Biosynthesis by Oxidative Stress in Acetate-Induced Cyst Cells of a Green Unicellular Alga, Haematococcus pluvialis

Survival of nonculturable Aeromonas salmonicida in lake water.

Viability and Isolation of Marine Bacteria by Dilution Culture: Theory, Procedures, and Initial Results

Introduction and expression of the bacterial genes cysE and cysK in eukaryotic cells.

Differential Responses of Nitrogen-Fixing Cyanobacteria to Salinity and Osmotic Stresses

Flow Cytometric Analysis of Marine Bacteria with Hoechst 33342 †

Hydroxydiether Lipid Structures in Methanosarcina spp. and Methanococcus voltae †

Direct extraction and purification of rRNA for ecological studies.

Differences in the hybridization pattern of Bacillus subtilis genes coding for rDNA depend on the method of DNA preparation.

Quality control of bacterial enumeration.

Effects of Growth Pressure and Temperature on Fatty Acid Composition of a Barotolerant Deep-Sea Bacterium

Purification, characterization, gene cloning, and sequencing of a new beta-glucosidase from Bacillus circulans subsp. alkalophilus.

Diversity within a Colony Morphotype: Implications for Ecological Research

Comparison of fluorescent gentamicin-thallous-carbonate and KF streptococcal agars to enumerate enterococci and fecal streptococci in meats.

Chromosome Polymorphisms among Strains of Hansenula polymorpha (syn. Pichia angusta)

Comparison of chemical assay, bioassay, enzyme-linked immunosorbent assay, and dot blot hybridization for detection of aerobactin in members of the family Enterobacteriaceae.

Year: 1993, Volume: 59, Issue: 2

Year: 1993, Volume: 59, Issue: 1

Year: 1992, Volume: 58

Year: 1991, Volume: 57

Year: 1990, Volume: 56

Year: 1989, Volume: 55

Year: 1988, Volume: 54

Year: 1987, Volume: 53

Year: 1986, Volume: 52

Year: 1986, Volume: 51

Year: 1985, Volume: 50

Year: 1985, Volume: 49

Year: 1984, Volume: 48

Year: 1984, Volume: 47

Year: 1983, Volume: 46

Year: 1983, Volume: 45

Year: 1982, Volume: 44

Year: 1982, Volume: 43

Year: 1981, Volume: 42

Year: 1981, Volume: 41

Year: 1980, Volume: 40

Year: 1980, Volume: 39

Year: 1979, Volume: 38

Year: 1979, Volume: 37

Year: 1978, Volume: 36

Year: 1978, Volume: 35

Year: 1977, Volume: 34

Year: 1977, Volume: 33

Year: 1976, Volume: 32

Year: 1976, Volume: 31

Viability and Isolation of Marine Bacteria by Dilution Culture: Theory, Procedures, and Initial Results

Content Provider	PubMed Central
Author	Button, D. K. Schut, Frits Quang, Pham Martin, Ravonna Robertson, Betsy R.
Abstract	Dilution culture, a method for growing the typical small bacteria from natural aquatic assemblages, has been developed. Each of 11 experimental trials of the technique was successful. Populations are measured, diluted to a small and known number of cells, inoculated into unamended sterilized seawater, and examined three times for the presence of 104 or more cells per ml over a 9-week interval. Mean viability for assemblage members is obtained from the frequency of growth, and many of the cultures produced are pure. Statistical formulations for determining viability and the frequency of pure culture production are derived. Formulations for associated errors are derived as well. Computer simulations of experiments agreed with computed values within the expected error, which verified the formulations. These led to strategies for optimizing viability determinations and pure culture production. Viabilities were usually between 2 and 60% and decreased with >5 mg of amino acids per liter as carbon. In view of difficulties in growing marine oligobacteria, these high values are noteworthy. Significant differences in population characteristics during growth, observed by high-resolution flow cytometry, suggested substantial population diversity. Growth of total populations as well as of cytometry-resolved subpopulations sometimes were truncated at levels of near 104 cells per ml, showing that viable cells could escape detection. Viability is therefore defined as the ability to grow to that population; true viabilities could be even higher. Doubling times, based on whole populations as well as individual subpopulations, were in the 1-day to 1-week range. Data were examined for changes in viability with dilution suggesting cell-cell interactions, but none could be confirmed. The frequency of pure culture production can be adjusted by inoculum size if the viability is known. These apparently pure cultures produced retained the size and apparent DNA-content characteristic of the bulk of the organisms in the parent seawater. Three cultures are now available, two of which have been carried for 3 years. The method is thus seen as a useful step for improving our understanding of typical aquatic organisms.
Starting Page	881
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	3
Volume Number	59
Language	English
Publisher Date	1993-03-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in