NDLI: Comparative study on the production of guar alpha-galactosidase by Saccharomyces cerevisiae SU50B and Hansenula polymorpha 8/2 in continuous cultures.

Please wait, while we are loading the content...

Year: 2016, Volume: 82

Year: 2015, Volume: 82

Year: 2015, Volume: 81

Year: 2014, Volume: 80

Year: 2013, Volume: 79

Year: 2012, Volume: 78

Year: 2011, Volume: 77

Year: 2010, Volume: 76

Year: 2009, Volume: 75

Year: 2008, Volume: 74

Year: 2007, Volume: 73

Year: 2006, Volume: 72

Year: 2005, Volume: 71

Year: 2004, Volume: 70

Year: 2003, Volume: 69

Year: 2002, Volume: 69

Year: 2002, Volume: 68

Year: 2001, Volume: 67

Year: 2000, Volume: 66

Year: 1999, Volume: 65

Year: 1998, Volume: 64

Year: 1997, Volume: 63

Year: 1996, Volume: 62

Year: 1995, Volume: 61

Year: 1994, Volume: 60

Year: 1993, Volume: 59

Year: 1993, Volume: 59, Issue: 12

Year: 1993, Volume: 59, Issue: 11

Year: 1993, Volume: 59, Issue: 10

Year: 1993, Volume: 59, Issue: 9

Year: 1993, Volume: 59, Issue: 8

Year: 1993, Volume: 59, Issue: 7

Year: 1993, Volume: 59, Issue: 6

Year: 1993, Volume: 59, Issue: 5

Year: 1993, Volume: 59, Issue: 4

Year: 1993, Volume: 59, Issue: 3

Year: 1993, Volume: 59, Issue: 2

Year: 1993, Volume: 59, Issue: 1

Identification and ecology of bacterial communities associated with necroses of three cactus species.

Precipitation of cadmium by Clostridium thermoaceticum.

Administration of different Lactobacillus strains in fermented oatmeal soup: in vivo colonization of human intestinal mucosa and effect on the indigenous flora.

Insertion of Transposon Tn917 Derivatives into the Lactococcus lactis subsp. lactis Chromosome

Formation and Regeneration of Methanococcus voltae Protoplasts †

Inhibition of adhesion of Escherichia coli K88 to piglet ileal mucus by Lactobacillus spp.

Evidence for catabolite inhibition in regulation of pentose utilization and transport in the ruminal bacterium Selenomonas ruminantium.

Benthic Distribution of Sewage Sludge Indicated by Clostridium perfringens at a Deep-Ocean Dump Site †

Comparative study on the production of guar alpha-galactosidase by Saccharomyces cerevisiae SU50B and Hansenula polymorpha 8/2 in continuous cultures.

Growth, copper-tolerant cells, and extracellular protein production in copper-stressed chemostat cultures of Vibrio alginolyticus.

Waterborne Giardia cysts and Cryptosporidium oocysts in the Yukon, Canada.

Antagonistic Effect of Nonpathogenic Fusarium oxysporum Fo47 and Pseudobactin 358 upon Pathogenic Fusarium oxysporum f. sp. dianthi

Isolation and purification of propionicin PLG-1, a bacteriocin produced by a strain of Propionibacterium thoenii.

Effects of Calcium on Sugar Transport in Azotobacter vinelandii †

Organic Thiols as Organolithotrophic Substrates for Growth of Phototrophic Bacteria

Characterization of the genome of the Rhodococcus rhodochrous bacteriophage NJL.

Bacterial Disproportionation of Elemental Sulfur Coupled to Chemical Reduction of Iron or Manganese

Influence of Complex Structure on the Biodegradation of Iron-Citrate Complexes

Glucose Uptake and End Product Formation in an Intertidal Marine Sediment †

Inoculation of Woody Legumes with Selected Arbuscular Mycorrhizal Fungi and Rhizobia To Recover Desertified Mediterranean Ecosystems

Comparison of the post-Chernobyl 137Cs contamination of mushrooms from eastern Europe, Sweden, and North America.

Detection and distribution of rotavirus in raw sewage and creeks in São Paulo, Brazil.

Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization.

Characterization of RFRS9, a second member of the Rhizobium fredii repetitive sequence family from the nitrogen-fixing symbiont R. fredii USDA257.

Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus.

Occurrence of UV-Absorbing, Mycosporine-Like Compounds among Cyanobacterial Isolates and an Estimate of Their Screening Capacity

Evidence Regarding the UV Sunscreen Role of a Mycosporine-Like Compound in the Cyanobacterium Gloeocapsa sp

Characterization of a Cell Envelope-Associated Proteinase Activity from Streptococcus thermophilus H-Strains

Adaptation and growth of Serratia marcescens in contact lens disinfectant solutions containing chlorhexidine gluconate.

Regulation and cloning of the gene encoding amylase activity of the ruminal bacterium Streptococcus bovis.

Restriction/Modification Systems and Restriction Endonucleases Are More Effective on Lactococcal Bacteriophages That Have Emerged Recently in the Dairy Industry †

Differentiation of Two Abortive Mechanisms by Using Monoclonal Antibodies Directed toward Lactococcal Bacteriophage Capsid Proteins †

Properties of nisin Z and distribution of its gene, nisZ, in Lactococcus lactis.

Monitoring airborne fungal spores in an experimental indoor environment to evaluate sampling methods and the effects of human activity on air sampling.

Production of (R)-3-Chloro-1,2-Propanediol from Prochiral 1,3-Dichloro-2-Propanol by Corynebacterium sp. Strain N-1074

Characterization of Ethanol Production from Xylose and Xylitol by a Cell-Free Pachysolen tannophilus System

Production of Extracellular Proteins by the Biocontrol Fungus Gliocladium virens

Evaluation of the Biolog Substrate Utilization System To Identify and Assess Metabolic Variation among Strains of Xanthomonas campestris pv. Citri

Characterization of the structural gene encoding a copper-containing nitrite reductase and homology of this gene to DNA of other denitrifiers.

Presence of lactate dehydrogenase and lactate racemase in Megasphaera elsdenii grown on glucose or lactate.

Manganese Peroxidase, Produced by Trametes versicolor during Pulp Bleaching, Demethylates and Delignifies Kraft Pulp

Kraft Pulp Bleaching and Delignification by Dikaryons and Monokaryons of Trametes versicolor

Molecular analysis of the rolling-circle replicating plasmid pA1 of Lactobacillus plantarum A112.

Stereospecificity of Microbial Hydrations of Oleic Acid to 10-Hydroxystearic Acid

Microbial degradation of dibenzofuran, fluorene, and dibenzo-p-dioxin by Staphylococcus auriculans DBF63.

Cobalamin-mediated mercury methylation by Desulfovibrio desulfuricans LS.

Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system.

Differentiation of Listeria monocytogenes and Listeria innocua by 16S rRNA genes and intraspecies discrimination of Listeria monocytogenes strains by random amplified polymorphic DNA polymorphisms.

Biological Control of Agrobacterium tumefaciens, Colonization, and pAgK84 Transfer with Agrobacterium radiobacter K84 and the Tra- Mutant Strain K1026

Strains of Corynebacterium glutamicum with Different Lysine Productivities May Have Different Lysine Excretion Systems

Chromosomal rearrangements during vegetative growth of a wild strain of Saccharomyces cerevisiae.

Quantitative observations of heterogeneities in Pseudomonas aeruginosa biofilms.

Cloning and sequencing of pepC, a cysteine aminopeptidase gene from Lactococcus lactis subsp. cremoris AM2.

Construction and characterization of heavy metal-resistant haloaromatic-degrading Alcaligenes eutrophus strains.

Starvation-survival of a p-nitrophenol-degrading bacterium.

Comparison of membrane filters for recovery of legionellae from water samples.

Cloning and characterization of the gene encoding glutamate 1-semialdehyde 2,1-aminomutase, which is involved in delta-aminolevulinic acid synthesis in Propionibacterium freudenreichii.

Stereospecific Hydroxylation of Indan by Escherichia coli Containing the Cloned Toluene Dioxygenase Genes from Pseudomonas putida F1

Bacterioplankton Community Structure and Dynamics after Large-Scale Release of Nonindigenous Bacteria as Revealed by Low-Molecular-Weight-RNA Analysis

Culture and Decontamination Methods Affecting Enumeration of Phages Infecting Bacteroides fragilis in Sewage

Year: 1992, Volume: 58

Year: 1991, Volume: 57

Year: 1990, Volume: 56

Year: 1989, Volume: 55

Year: 1988, Volume: 54

Year: 1987, Volume: 53

Year: 1986, Volume: 52

Year: 1986, Volume: 51

Year: 1985, Volume: 50

Year: 1985, Volume: 49

Year: 1984, Volume: 48

Year: 1984, Volume: 47

Year: 1983, Volume: 46

Year: 1983, Volume: 45

Year: 1982, Volume: 44

Year: 1982, Volume: 43

Year: 1981, Volume: 42

Year: 1981, Volume: 41

Year: 1980, Volume: 40

Year: 1980, Volume: 39

Year: 1979, Volume: 38

Year: 1979, Volume: 37

Year: 1978, Volume: 36

Year: 1978, Volume: 35

Year: 1977, Volume: 34

Year: 1977, Volume: 33

Year: 1976, Volume: 32

Year: 1976, Volume: 31

Comparative study on the production of guar alpha-galactosidase by Saccharomyces cerevisiae SU50B and Hansenula polymorpha 8/2 in continuous cultures.

Content Provider	PubMed Central
Author	Giuseppin, M. L. Almkerk, J. W. Heistek, J. C. Verrips, C. T.
Abstract	Saccharomyces cerevisiae SU50B and Hansenula polymorpha 8/2, both carrying a multicopy integrated guar alpha-galactosidase, have been cultivated in continuous cultures, using various mixtures of carbon sources and cultivation conditions. Both S. cerevisiae SU50B and H. polymorpha 8/2 are stable and produce high levels of extracellular alpha-galactosidase in continuous cultures for more than 500 h. For these expression systems the strong inducible promoter systems GAL7 and methanol oxidase, respectively, were used. The induction of alpha-galactosidase synthesis by galactose in SU50B is limited by the low galactose uptake. Apart from that, at high dilution rates, the glucose repression is substantial, and a maximal expression level of 28.6 mg of extracellular alpha-galactosidase.g (dry weight) of biomass-1 could be obtained. In H. polymorpha, the induction of alpha-galactosidase synthesis, in addition to methanol oxidase synthesis using formaldehyde, is very effective up to 42 mg of extracellular alpha-galactosidase.g (dry weight) of biomass-1. Productivities in terms of specific production rate enable a good comparison with those of other heterologous expression systems in the literature. The productivities found with S. cerevisiae SU50B and H. polymorpha, 3.25 and 5.5 mg of alpha-galactosidase.g of biomass-1.liter-1.h-1, respectively, rank among the highest reported in the literature. Enzyme production and secretion in H. polymorpha are more complex. A two-peaked optimum is found in enzyme production. No clear explanation of this phenomenon can be given.
Starting Page	52
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	1
Volume Number	59
Language	English
Publisher Date	1993-01-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in