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Improvement of pancreatic islet cell isolation for transplantation

Content Provider	PubMed Central
Author	Matsumoto, Shinichi Noguchi, Hirofumi Naziruddin, Bashoo Nicolas, Onaca Jackson, Andrew Hatanaka, Nobuyo Okitsu, Teru Kobayashi, Naoya Klintmalm, Göran Levy, Marlon
Copyright Year	2007
Abstract	Pancreatic islet transplantation is a promising treatment for diabetes but still faces several challenges. Poor islet isolation efficiency and poor long-term insulin independence are currently two major issues, although donor shortage and the need for immunosuppressants also need to be addressed. We established the Kyoto islet isolation method (KIIM), which has enabled us to isolate and transplant islets even from non–heart-beating donors. KIIM involves 1) cooling the donor pancreas in situ, 2) preserving the ducts with modified Kyoto solution, 3) using a modified two-layer pancreas preservation method, and 4) adjusting the density of the density gradient centrifugation and using an iodixanol-based solution for purification. KIIM has enabled us to transplant 17 islet preparations out of 21 isolations (an 81% success rate). All transplanted islets functioned, and all transplanted patients had improved glycemic control without hypoglycemic unawareness. Recently, we used KIIM for islet isolation from a brain-dead donor at Baylor, which resulted in a very high islet yield (789,984 IE) with high viability (100% by fluorescein diacetate/propidium iodide staining and a stimulation index of 4.7). This preliminary evidence suggests that KIIM may also be promising for islet isolation from brain-dead donors. In addition, to assess engrafted islet mass, we developed a secretory unit of islet transplant objects (SUITO) index: fasting C-peptide (ng/dL) / [fasting blood glucose (mg/dL) – 63] × 1500. This simple index has enabled us to monitor the engrafted islet mass. This index should be useful when deciding whether to perform additional islet transplantations to maintain insulin independence. Poor islet isolation efficacy and poor long-term results could be resolved with ongoing research.
Starting Page	357
File Format	PDF
ISSN	08998280
Journal	Proceedings (Baylor University. Medical Center)
Issue Number	4
Volume Number	20
Language	English
Publisher	Baylor Health Care System
Publisher Date	2007-10-01
Access Restriction	Open
Rights Holder	Baylor Health Care System
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine

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