NDLI: Anaerobic degradation of toluene and o-xylene by a methanogenic consortium.

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Characterization of psychrotrophic microorganisms producing beta-galactosidase activities.

Isolation of Bacillus thuringiensis from Stored Tobacco and Lasioderma serricorne (F.)

Electrophoretic Transfer from Polyacrylamide Gel to Nitrocellulose Sheets, a New Method To Characterize Multilocus Enzyme Genotypes of Klebsiella Strains

Structural and immunological characterization of a biosurfactant produced by Bacillus licheniformis JF-2.

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Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR.

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Quantitative Centrifugation To Extract Benthic Protozoa from Freshwater Sediments

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Evidence of a dormant but infective state of the fish pathogen Pasteurella piscicida in seawater and sediment.

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Evaluation of Data Transformations and Validation of a Model for the Effect of Temperature on Bacterial Growth

Modeling of Bacterial Growth with Shifts in Temperature

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Characterization of Microbial Community Structure in the Surface Sediment of Osaka Bay, Japan, by Phospholipid Fatty Acid Analysis

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Identification and characterization of a new plasmid carrying genes for degradation of 2,4-dichlorophenoxyacetate from Pseudomonas cepacia CSV90.

Anaerobic degradation of toluene and o-xylene by a methanogenic consortium.

A method for detection of aromatic metabolites at very low concentrations: application to detection of metabolites of anaerobic toluene degradation.

Changes in Bacterial Numbers and Leucine Assimilation during Estimations of Microbial Respiratory Rates in Seawater by the Precision Winkler Method

Characterization of the Lactobacillus helveticus CNRZ32 pepC gene.

Heterologous expression of endo-beta-1,4-D-glucanase from Clostridium cellulovorans in Clostridium acetobutylicum ATCC 824 following transformation of the engB gene.

Characterization of an endoserine protease secreted by Arthrobacter aureus.

Inactivation of Phage MS2 by Iron-Aided Titanium Dioxide Photocatalysis

Transcriptional enhancement of the Listeria monocytogenes PCR and simple immunoenzymatic assay of the product using anti-RNA:DNA antibodies.

PCR analysis of the cryI insecticidal crystal family genes from Bacillus thuringiensis.

Pseudomonas putida Strains Which Constitutively Overexpress Mercury Resistance for Biodetoxification of Organomercurial Pollutants

Chemotaxis to oligopeptides by Pseudomonas aeruginosa.

Detection of Salmonella spp. in oysters by PCR.

Use of solid-phase PCR for enhanced detection of airborne microorganisms.

Isolation of an insertion sequence (IS1051) from Xanthomonas campestris pv. dieffenbachiae with potential use for strain identification and characterization.

Microbial Degradation of Poly(3-Hydroxybutyrate) and Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) in Soils

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Anaerobic degradation of toluene and o-xylene by a methanogenic consortium.

Content Provider	PubMed Central
Author	Edwards, E. A. Grbić-galić, D.
Abstract	Toluene and o-xylene were completely mineralized to stoichiometric amounts of carbon dioxide, methane, and biomass by aquifer-derived microorganisms under strictly anaerobic conditions. The source of the inoculum was creosote-contaminated sediment from Pensacola, Fla. The adaptation periods before the onset of degradation were long (100 to 120 days for toluene degradation and 200 to 255 days for o-xylene). Successive transfers of the toluene- and o-xylene-degrading cultures remained active. Cell density in the cultures progressively increased over 2 to 3 years to stabilize at approximately 10(9) cells per ml. Degradation of toluene and o-xylene in stable mixed methanogenic cultures followed Monod kinetics, with inhibition noted at substrate concentrations above about 700 microM for o-xylene and 1,800 microM for toluene. The cultures degraded toluene or o-xylene but did not degrade m-xylene, p-xylene, benzene, ethylbenzene, or naphthalene. The degradative activity was retained after pasteurization or after starvation for 1 year. Degradation of toluene and o-xylene was inhibited by the alternate electron acceptors oxygen, nitrate, and sulfate. Degradation was also inhibited by the addition of preferred substrates such as acetate, H2, propionate, methanol, acetone, glucose, amino acids, fatty acids, peptone, and yeast extract. These data suggest that the presence of natural organic substrates or contaminants may inhibit anaerobic degradation of pollutants such as toluene and o-xylene at contaminated sites.
Starting Page	313
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	1
Volume Number	60
Language	English
Publisher Date	1994-01-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

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