NDLI: Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion

Content Provider	PubMed Central
Author	Degtyar, Vadim E. Allersma, Miriam W. Axelrod, Daniel Holz, Ronald W.
Copyright Year	2007
Abstract	The state of secretory granules immediately before fusion with the plasma membrane is unknown, although the granules are generally assumed to be stably bound (docked). We had previously developed methods using total internal reflection fluorescence microscopy and image analysis to determine the position of chromaffin granules immediately adjacent to the plasma membrane with high precision, often to within ≈10 nm, or <5% of the granule diameter (300 nm). These distances are of the dimensions of large proteins and are comparable with the unitary step sizes of molecular motors. Here we demonstrate with quantitative measures of granule travel in the plane parallel to the plasma membrane that secretory granules change position within several hundred milliseconds of nicotinic agonist-induced fusion. Furthermore, just before fusion, granules frequently move to areas that they have rarely visited. The movement of granules to new areas is most evident for granules that fuse later during the stimulus. The movement may increase the probability of productive interactions of the granule with the plasma membrane or may reflect the pull of molecular interactions between the granule and the plasma membrane that are part of the fusion process. Thus, instead of being stably docked before exocytosis, granules undergo molecular-scale motions and travel immediately preceding the fusion event.
Related Links	http://dx.doi.org/10.1073/pnas.0705406104
Ending Page	15934
Page Count	6
Starting Page	15929
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	40
Volume Number	104
Language	English
Publisher	National Academy of Sciences
Publisher Date	2007-10-02
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion.

The last few milliseconds in the life of a secretory granule : Docking, dynamics and fusion visualized by total internal reflection fluorescence microscopy (TIRFM)

Two-year-olds with autism orient to nonsocial contingencies rather than biological motion

Tension in secretory granule membranes causes extensive membrane transfer through the exocytotic fusion pore.

Tension in secretory granule membranes causes extensive membrane transfer through the exocytotic fusion pore

A germ-line-selective advantage rather than an increased mutation rate can explain some unexpectedly common human disease mutations

Increased secretory demand rather than a defect in the proinsulin conversion mechanism causes hyperproinsulinemia in a glucose-infusion rat model of non-insulin-dependent diabetes mellitus.

TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic β-cells: different behaviour of granule motion between normal and Goto–Kakizaki diabetic rat β-cells

Lack of phylogeography in European mammals before the last glaciation

Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion

Similar Documents

Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion.

The last few milliseconds in the life of a secretory granule : Docking, dynamics and fusion visualized by total internal reflection fluorescence microscopy (TIRFM)

Two-year-olds with autism orient to nonsocial contingencies rather than biological motion

Tension in secretory granule membranes causes extensive membrane transfer through the exocytotic fusion pore.

Tension in secretory granule membranes causes extensive membrane transfer through the exocytotic fusion pore

A germ-line-selective advantage rather than an increased mutation rate can explain some unexpectedly common human disease mutations

Increased secretory demand rather than a defect in the proinsulin conversion mechanism causes hyperproinsulinemia in a glucose-infusion rat model of non-insulin-dependent diabetes mellitus.

TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic β-cells: different behaviour of granule motion between normal and Goto–Kakizaki diabetic rat β-cells

Lack of phylogeography in European mammals before the last glaciation

Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion