NDLI: Uptake Hydrogenase (Hup) in Common Bean (Phaseolus vulgaris) Symbioses

Content Provider	PubMed Central
Author	Navarro, Rosangela B. Vargas, Alvaro A. T. Schröder, Eduardo C. Berkum, Peter Van
Abstract	Strains of Rhizobium forming nitrogen-fixing symbioses with common bean were systematically examined for the presence of the uptake hydrogenase (hup) structural genes and expression of uptake hydrogenase (Hup) activity. DNA with homology to the hup structural genes of Bradyrhizobium japonicum was present in 100 of 248 strains examined. EcoRI fragments with molecular sizes of approximately 20.0 and 2.2 kb hybridized with an internal SacI fragment, which contains part of both bradyrhizobial hup structural genes. The DNA with homology to the hup genes was located on pSym of one of the bean rhizobia. Hup activity was observed in bean symbioses with 13 of 30 strains containing DNA homologous with the hup structural genes. However, the Hup activity was not sufficient to eliminate hydrogen evolution from the nodules. Varying the host plant with two of the Hup+ strains indicated that expression of Hup activity was host regulated, as has been reported with soybean, pea, and cowpea strains.
Starting Page	4161
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	12
Volume Number	59
Language	English
Publisher Date	1993-12-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

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Uptake Hydrogenase (Hup) in Common Bean (Phaseolus vulgaris) Symbioses