NDLI: Evaluation of aquatic sediment microcosms and their use in assessing possible effects of introduced microorganisms on ecosystem parameters.

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Purification and characterization of an extracellular muramidase of Clostridium acetobutylicum ATCC 824 that acts on non-N-acetylated peptidoglycan.

Purification and Characterization of an α-l-Arabinofuranosidase from Butyrivibrio fibrisolvens GS113

Extracellular Polysaccharide Is Not Responsible for Aluminum Tolerance of Rhizobium leguminosarum bv. Phaseoli CIAT899

Redox Cycling of Iron Supports Growth and Magnetite Synthesis by Aquaspirillum magnetotacticum

Anaerobic dechlorination of polychlorobiphenyls (Aroclor 1242) by pasteurized and ethanol-treated microorganisms from sediments.

Effect of pH and Monensin on Glucose Transport by Fibrobacter succinogenes, a Cellulolytic Ruminal Bacterium

Lignocellulose Degradation during Solid-State Fermentation: Pleurotus ostreatus versus Phanerochaete chrysosporium

Conversion of xylan to ethanol by ethanologenic strains of Escherichia coli and Klebsiella oxytoca.

Regulation of Proteolytic Activity in the Hyperthermophile Pyrococcus furiosus

Differential bioavailability of soil-sorbed naphthalene to two bacterial species.

Chemotaxis of Rhizobium meliloti towards Nodulation Gene-Inducing Compounds from Alfalfa Roots

Use of Hydrogen Peroxide Treatment and Crystal Violet Agar Plates for Selective Recovery of Bacteriophages from Natural Environments †

Microsensor Measurements of Sulfate Reduction and Sulfide Oxidation in Compact Microbial Communities of Aerobic Biofilms

Corrosion and Electrochemical Oxidation of a Pyrite by Thiobacillus ferrooxidans

Degradation of hydrogen sulfide by Xanthomonas sp. strain DY44 isolated from peat.

Localization of Glucose Oxidase and Catalase Activities in Aspergillus niger

Physiological and nutritional factors affecting synthesis of extracellular metalloproteases by Clostridium bifermentans NCTC 2914.

Influence of Polyethylene Glycol on the Size of Schizosaccharomyces pombe Electropores

Efficacy of burning, tillage, and biocides in controlling bacteria released at field sites and effects on indigenous bacteria and fungi.

Glyphosate degradation by immobilized bacteria: field studies with industrial wastewater effluent.

Aerobic vinyl chloride metabolism in Mycobacterium aurum L1.

Self-similar colony morphogenesis by gram-negative rods as the experimental model of fractal growth by a cell population.

Parameters Affecting Solvent Production by Clostridium pasteurianum

Efficacies of Novel N-Halamine Disinfectants against Salmonella and Pseudomonas Species

Immunomagnetically Captured Thermophilic Sulfate-Reducing Bacteria from North Sea Oil Field Waters

Evaluation of aquatic sediment microcosms and their use in assessing possible effects of introduced microorganisms on ecosystem parameters.

Survival and function of a genetically engineered Pseudomonad in aquatic sediment microcosms.

Changes in Molecular Size Distribution of Cellulose during Attack by White Rot and Brown Rot Fungi

Polymerase chain reaction and gene probe detection of the 2,4-dichlorophenoxyacetic acid degradation plasmid, pJP4.

Isolation of Pseudomonas pickettii strains that degrade 2,4,6-trichlorophenol and their dechlorination of chlorophenols.

Relationship between Desiccation and Exopolysaccharide Production in a Soil Pseudomonas sp

Isolation and screening of plasmids from the epilithon which mobilize recombinant plasmid pD10.

Growth Characteristics of the Thermophilic Fungus Scytalidium thermophilum in Relation to Production of Mushroom Compost

Complete oxidation of linear alkylbenzene sulfonate by bacterial communities selected from coastal seawater.

Evidence for natural transfer of a tetracycline resistance gene between bacteria from the human colon and bacteria from the bovine rumen.

Toxin Production by Microcystis aeruginosa as a Function of Light in Continuous Cultures and Its Ecological Significance

Physicochemical and structural investigation of the surfaces of some anaerobic subgingival bacteria.

Influence of pH, Oxygen, and Humic Substances on Ability of Sunlight To Damage Fecal Coliforms in Waste Stabilization Pond Water

Distribution, Frequency, and Diversity of Bacillus thuringiensis in an Animal Feed Mill

Metabolism of phenanthrene by the marine cyanobacterium Agmenellum quadruplicatum PR-6.

Evidence for an NIH shift in oxidation of naphthalene by the marine cyanobacterium Oscillatoria sp. strain JCM.

Metabolism of Phosphonoacetate as the Sole Carbon and Phosphorus Source by an Environmental Bacterial Isolate

Reductive dehalogenation of chlorophenols by Desulfomonile tiedjei DCB-1.

Survival and replication of male-specific bacteriophages in molluscan shellfish.

Bacterial Hydrolysis of Protein and Methylated Protein and Its Implications for Studies of Protein Degradation in Aquatic Systems

Conditions for protoplasting, regenerating, and transforming the calicheamicin producer, Micromonospora echinospora.

Method to identify specific alleles of a Phanerochaete chrysosporium gene encoding lignin peroxidase.

Efficient fermentation of Pinus sp. acid hydrolysates by an ethanologenic strain of Escherichia coli.

Enzymic Dehalogenation of 4-Chlorobenzoyl Coenzyme A in Acinetobacter sp. Strain 4-CB1

Use of a genetically engineered Escherichia coli strain to produce 1,2-dihydroxy-4'-chlorobiphenyl.

Stimulation of Reductive Dechlorination of Tetrachloroethene in Anaerobic Aquifer Microcosms by Addition of Short-Chain Organic Acids or Alcohols

Factors Stimulating Propagation of Legionellae in Cooling Tower Water

Methanogenic Degradation of Poly(3-Hydroxyalkanoates)

Cloning, expression, and nucleotide sequence of a lipase gene from Pseudomonas fluorescens B52.

Bdellovibrios in Callinectus sapidus, the Blue Crab

Sporulation and enterotoxin production by Clostridium perfringens type A at 37 and 43 degrees C.

Variation in Microbial Biomass and Community Structure in Sediments of Eutrophic Bays as Determined by Phospholipid Ester-Linked Fatty Acids

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Evaluation of aquatic sediment microcosms and their use in assessing possible effects of introduced microorganisms on ecosystem parameters.

Content Provider	PubMed Central
Author	Pipke, R. Timmis, K. N. Dwyer, D. F. Wagner-Döbler, I.
Abstract	In this paper we describe a sediment microcosm system consisting of 20 undisturbed, layered sediment cores with overlying site water which are incubated under identical conditions of temperature, light, stirring rate of overlying water, and water exchange rate. Ecosystem parameters (nutrient level, photosynthetic potential, community structure of heterotrophic bacteria, thymidine incorporation rate, and oxygen microgradients) of the laboratory microcosms and the source ecosystem were compared and shown to be indistinguishable for the first 2 weeks. In weeks 3 and 4, small differences were detectable in the nutrient level, community structure of heterotrophic bacteria, and thymidine incorporation rate. However, the photosynthetic potential, depth profiles of heterotrophic bacterial community structure, and oxygen microgradients were maintained throughout the incubation period and did not differ between laboratory microcosms and the source ecosystem. The microcosm system described here would thus appear to be a valid model of aquatic sediments for up to 4 weeks; the actual period would depend on the sediment source and incubation temperature. The validated systems were used with Rhine river sediment to assess possible effects on ecosystem parameters of Pseudomonas sp. strain B13 FR1(pFRC20P), a genetically engineered microorganism (GEM) that had been constructed to degrade mixtures of halo- and alkylbenzoates and -phenols. The GEM survived in the surface sediment at densities of 5 x 10(4) to 5 x 10(5)/g (dry weight) for 4 weeks and degraded added chloro- and methylaromatics. The GEM did not measurably influence ecosystem parameters such as photosynthesis, densities of selected heterotrophic bacteria, thymidine incorporation rate, and oxygen microgradients. Thus, the microcosm system described here would seem to be useful for the study of the ecology of biodegradation and the fate and effect of microorganisms introduced into the environment.
Starting Page	1249
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	4
Volume Number	58
Language	English
Publisher Date	1992-04-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

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