NDLI: Development and Use of Field Application Vectors To Express Nonadaptive Foreign Genes in Competitive Environments †

Please wait, while we are loading the content...

Year: 2016, Volume: 82

Year: 2015, Volume: 82

Year: 2015, Volume: 81

Year: 2014, Volume: 80

Year: 2013, Volume: 79

Year: 2012, Volume: 78

Year: 2011, Volume: 77

Year: 2010, Volume: 76

Year: 2009, Volume: 75

Year: 2008, Volume: 74

Year: 2007, Volume: 73

Year: 2006, Volume: 72

Year: 2005, Volume: 71

Year: 2004, Volume: 70

Year: 2003, Volume: 69

Year: 2002, Volume: 69

Year: 2002, Volume: 68

Year: 2001, Volume: 67

Year: 2000, Volume: 66

Year: 1999, Volume: 65

Year: 1998, Volume: 64

Year: 1997, Volume: 63

Year: 1996, Volume: 62

Year: 1995, Volume: 61

Year: 1994, Volume: 60

Year: 1993, Volume: 59

Year: 1992, Volume: 58

Year: 1992, Volume: 58, Issue: 12

Year: 1992, Volume: 58, Issue: 11

Year: 1992, Volume: 58, Issue: 10

Year: 1992, Volume: 58, Issue: 9

Year: 1992, Volume: 58, Issue: 8

Year: 1992, Volume: 58, Issue: 7

Year: 1992, Volume: 58, Issue: 6

Year: 1992, Volume: 58, Issue: 5

Year: 1992, Volume: 58, Issue: 4

Year: 1992, Volume: 58, Issue: 3

Year: 1992, Volume: 58, Issue: 2

Microbial reduction of manganese and iron: new approaches to carbon cycling.

Cell surface redox potential as a mechanism of defense against photosensitizers in fungi.

Enzymatic iron oxidation by Leptothrix discophora: identification of an iron-oxidizing protein.

Molecular cloning of genes related to aflatoxin biosynthesis by differential screening.

Effect of different holding regimens on the intestinal microflora of herring (Clupea harengus) larvae.

Identification of two proline transport systems in Staphylococcus aureus and their possible roles in osmoregulation.

Development of a lactococcal integration vector by using IS981 and a temperature-sensitive lactococcal replication region.

Effects of chlorobenzoate transformation on the Pseudomonas testosteroni biphenyl and chlorobiphenyl degradation pathway.

Metabolites formed during anaerobic transformation of toluene and o-xylene and their proposed relationship to the initial steps of toluene mineralization.

Biodegradation of the herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) by purified pentachlorophenol hydroxylase and whole cells of Flavobacterium sp. strain ATCC 39723 is accompanied by cyanogenesis.

Properties of a 72-kilodalton mosquitocidal protein from Bacillus thuringiensis subsp. morrisoni PG-14 expressed in B. thuringiensis subsp. kurstaki by using the shuttle vector pHT3101.

Stromal Development and Mating System of Balansia epichloë, a Leaf-Colonizing Endophyte of Warm-Season Grasses

Isolation and partial characterization of an 87-kilodalton beta-1,3-glucanase from Bacillus circulans IAM1165.

Characterization of an extracellular protease inhibitor of Bacillus brevis HPD31 and nucleotide sequence of the corresponding gene.

DNA relatedness among strains of the sweet potato pathogen Streptomyces ipomoea (Person and Martin 1940) Waksman and Henrici 1948.

Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers.

Production of Concentrated Lactococcus lactis subsp. cremoris Suspensions in Calcium Alginate Beads

Intestinal colonization potential of turbot (Scophthalmus maximus)- and dab (Limanda limanda)-associated bacteria with inhibitory effects against Vibrio anguillarum.

Variation in Microbial Biomass and Community Structure in Sediments of Eutrophic Bays as Determined by Phospholipid Ester-Linked Fatty Acids

Cloning, sequencing, and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6.

Purification and Characterization of Extracellular Pectinolytic Enzymes Produced by Sclerotinia sclerotiorum

Measurement of marine picoplankton cell size by using a cooled, charge-coupled device camera with image-analyzed fluorescence microscopy.

Effects of Temperature on Two Psychrophilic Ecotypes of a Heterotrophic Nanoflagellate, Paraphysomonas imperforata

Bacterial Oxidation of Sulfide Minerals in Column Leaching Experiments at Suboptimal Temperatures

Formation of Novel Polysaccharides by Bradyrhizobium japonicum Bacteroids in Soybean Nodules

Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms.

Inhibition of Listeria monocytogenes by fatty acids and monoglycerides.

Characterization of Fluorescent Siderophore-Mediated Iron Uptake in Pseudomonas sp. Strain M114: Evidence for the Existence of an Additional Ferric Siderophore Receptor

Persistence of Viruses in Desert Soils Amended with Anaerobically Digested Sewage Sludge

Insecticidal properties of a crystal protein gene product isolated from Bacillus thuringiensis subsp. kenyae.

Construction of a 3-chlorobiphenyl-utilizing recombinant from an intergeneric mating.

Development and Use of Field Application Vectors To Express Nonadaptive Foreign Genes in Competitive Environments †

Reduction of Campylobacter jejuni colonization of chicks by cecum-colonizing bacteria producing anti-C. jejuni metabolites.

Total Degradation of EDTA by Mixed Cultures and a Bacterial Isolate

Use of monoclonal antibodies to demonstrate different sites with different functional characteristics in a bacterial lipase from Pseudomonas aeruginosa YS-7.

Disinfection of drinking water by using a novel electrochemical reactor employing carbon-cloth electrodes.

Light-dependent degradation of nitrophenols by the phototrophic bacterium Rhodobacter capsulatus E1F1.

Potential early intermediates in anaerobic benzoate degradation by Rhodopseudomonas palustris.

IS946-mediated integration of heterologous DNA into the genome of Lactococcus lactis subsp. lactis.

Comparison of acetate utilization among strains of an aceticlastic methanogen, Methanothrix soehngenii.

Genetic characterization of Legionella pneumophila serogroup 1 associated with respiratory disease in Australia.

Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri by pulsed-field gel electrophoresis.

Detection of Pediococcus spp. in brewing yeast by a rapid immunoassay.

Fluorimetric Detection of a Bacillus stearothermophilus Spore-Bound Enzyme, α-d-Glucosidase, for Rapid Indication of Flash Sterilization Failure

The Soybean Rj4 Allele Restricts Nodulation by Bradyrhizobium japonicum Serogroup 123 Strains †

Comparison of assimilable organic carbon and UV-oxidizable carbon for evaluation of ultrapure-water systems.

Preparation of Encapsulated Microbial Cells for Environmental Applications †

Production of an extracellular polyethylene-degrading enzyme(s) by Streptomyces species.

Effect of Yeast Extract and Vitamin B12 on Ethanol Production from Cellulose by Clostridium thermocellum I-1-B

Use of colistin-polymyxin B-cellobiose agar for isolation of Vibrio vulnificus from the environment.

Selective Medium for Quantitation of Bacillus popilliae in Soil and in Commercial Spore Powders

Effect of nitrogen limitation on long-side-chain poly-beta-hydroxyalkanoate synthesis by Pseudomonas resinovorans.

Filtration sizes of human immunodeficiency virus type 1 and surrogate viruses used to test barrier materials.

The plasmid-encoded lactococcal envelope-associated proteinase is encoded by a chromosomal gene in Lactococcus lactis subsp. cremoris BC101.

Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction.

Antifungal activity of n-tributyltin acetate against some common yam rot fungi.

Strain selection in carbon-limited chemostats affects reproducibility of Thermoanaerobacter ethanolicus fermentations.

Suitability of the prfA gene, which encodes a regulator of virulence genes in Listeria monocytogenes, in the identification of pathogenic Listeria spp.

Toxicity of some Fusarium section Sporotrichiella strains in relation to mycotoxin production.

Use of Inorganic Membrane Filters (Anopore) for Epifluorescence and Scanning Electron Microscopy of Nanoplankton and Picoplankton †

L. monocytogenes oligonucleotide probe.

Documentation of the claim that modulation of UV light pulses increases the bactericidal effectiveness of the light.

Occurrence of Giardia and Cryptosporidium spp. in Surface Water Supplies

Year: 1992, Volume: 58, Issue: 1

Year: 1991, Volume: 57

Year: 1990, Volume: 56

Year: 1989, Volume: 55

Year: 1988, Volume: 54

Year: 1987, Volume: 53

Year: 1986, Volume: 52

Year: 1986, Volume: 51

Year: 1985, Volume: 50

Year: 1985, Volume: 49

Year: 1984, Volume: 48

Year: 1984, Volume: 47

Year: 1983, Volume: 46

Year: 1983, Volume: 45

Year: 1982, Volume: 44

Year: 1982, Volume: 43

Year: 1981, Volume: 42

Year: 1981, Volume: 41

Year: 1980, Volume: 40

Year: 1980, Volume: 39

Year: 1979, Volume: 38

Year: 1979, Volume: 37

Year: 1978, Volume: 36

Year: 1978, Volume: 35

Year: 1977, Volume: 34

Year: 1977, Volume: 33

Year: 1976, Volume: 32

Year: 1976, Volume: 31

Development and Use of Field Application Vectors To Express Nonadaptive Foreign Genes in Competitive Environments †

Content Provider	PubMed Central
Author	Lajoie, C. A. Chen, S. Y. Oh, K. C. Strom, P. F.
Abstract	Many potential applications of genetically engineered microorganisms in environmental and agricultural biotechnology involve introducing genetic capabilities into nonsterile competitive environments in which they provide no advantage to the host. Field application vectors have been designed for the purpose of creating a temporary niche for the host in such environments. This technique involves the addition to the target environment of a selective substrate readily utilizable by the host microorganism but unavailable to most indigenous species. Thirteen nonionic and anionic detergents, representing a wide range of structural complexities and molecular weights, were screened as potential selective substrates. Competition experiments in soil, using Warburg respirometry, indicated that isolates from six different detergent enrichment cultures were more active on their corresponding detergents than the indigenous microorganisms. Detergents of intermediate structural complexities and molecular weights were most effective for use as selective substrates. A field application vector that utilizes 1.0% Igepal CO-720 (detergent) as the selective substrate and Pseudomonas paucimobilis 1IGP4 as the host was tested for its ability to increase the presence of nonadaptive tetracycline resistance marker genes in soil. In soil amended with the selective substrate, strain 1IGP4 plate counts increased by three orders of magnitude and tetracycline-resistant transformant (pRK293) counts increased from 1.8 × 106/g of soil to 4.3 × 108/g in 2 days. Inoculation in the absence of substrate amendment or amendment with a nonselective substrate did not result in growth of strain 1IGP4. These results demonstrate the effectiveness of field application vectors for increasing the concentration of nonadaptive genes in competitive environments.
Starting Page	655
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	2
Volume Number	58
Language	English
Publisher Date	1992-02-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in