NDLI: Enucleated L929 Cells Support Invasion, Differentiation, and Multiplication of Trypanosoma cruzi Parasites▿

Content Provider	PubMed Central
Author	Coimbra, Vanessa C. Yamamoto, Denise Khusal, Ketna G. Atayde, Vanessa Diniz Fernandes, Maria Cecília Mortara, Renato A. Yoshida, Nobuko Alves, Maria Julia M. Rabinovitch, Michel
Copyright Year	2007
Abstract	Cell infection with Trypanosoma cruzi, the agent of Chagas’ disease, begins with the uptake of infective trypomastigotes within phagosomes and their release into the cytosol, where they transform into replicating amastigotes; the latter, in turn, differentiate into cytolytically released and infective trypomastigotes. We ask here if the T. cruzi infection program can develop in enucleated host cells. Monolayers of L929 cells, enucleated by centrifugation in the presence of cytochalasin B and kept at 34°C to extend the survival of cytoplasts, were infected with parasites of the CL strain. Percent infection, morphology, stage-specific markers, and numbers of parasites per cell were evaluated in nucleated and enucleated cells, both of which were present in the same preparations. Parasite uptake, differentiation and multiplication of amastigotes, development of epimastigote- and trypomastigote-like forms, and initial cytolytic release of parasites were all documented for cytoplasts and nucleated cells. Although the doubling times were similar, parasite loads at 48 and 72 h were significantly lower in the cytoplasts than in nucleated cells. Similar results were obtained with the highly virulent strain Y as well as with strains CL-14 and G, which exhibit low virulence for mice. Cytoplasts could also be infected with the CL strain 24 or 48 h after enucleation. Thus, infection of cells by T. cruzi can take place in enucleated host cells, i.e., in the absence of modulation of chromosomal and nucleolar gene transcription and of RNA modification and processing in the nucleus.
Related Links	http://dx.doi.org/10.1128/iai.00194-07
Ending Page	3706
Page Count	7
Starting Page	3700
File Format	PDF
ISSN	00199567
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	8
Volume Number	75
Language	English
Publisher	American Society for Microbiology
Publisher Date	2007-08-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Immunology Microbiology Parasitology Infectious Diseases Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Involvement of Trypanosoma cruzi Metacyclic Trypomastigote Surface Molecule gp82 in Adhesion to Gastric Mucin and Invasion of Epithelial Cells

Anatomical Route of Invasion and Protective Mucosal Immunity in Trypanosoma cruzi Conjunctival Infection

Trypanosoma cruzi Induces Regulatory Dendritic Cells In Vitro▿

Fibronectin-Degrading Activity of Trypanosoma cruzi Cysteine Proteinase Plays a Role in Host Cell Invasion

A New Cruzipain-Mediated Pathway of Human Cell Invasion by Trypanosoma cruzi Requires Trypomastigote Membranes

Mammalian cell sialic acid enhances invasion by Trypanosoma cruzi.

Trypanosoma cruzi Infection Induces Proliferation of Vascular Smooth Muscle Cells

Mechanisms of invasion and replication of the intracellular stage in Trypanosoma cruzi.

Molecular Mechanisms of Host Cell Invasion by Trypanosoma cruzi

Enucleated L929 Cells Support Invasion, Differentiation, and Multiplication of Trypanosoma cruzi Parasites▿

Similar Documents

Involvement of Trypanosoma cruzi Metacyclic Trypomastigote Surface Molecule gp82 in Adhesion to Gastric Mucin and Invasion of Epithelial Cells

Anatomical Route of Invasion and Protective Mucosal Immunity in Trypanosoma cruzi Conjunctival Infection

Trypanosoma cruzi Induces Regulatory Dendritic Cells In Vitro▿

Fibronectin-Degrading Activity of Trypanosoma cruzi Cysteine Proteinase Plays a Role in Host Cell Invasion

A New Cruzipain-Mediated Pathway of Human Cell Invasion by Trypanosoma cruzi Requires Trypomastigote Membranes

Mammalian cell sialic acid enhances invasion by Trypanosoma cruzi.

Trypanosoma cruzi Infection Induces Proliferation of Vascular Smooth Muscle Cells

Mechanisms of invasion and replication of the intracellular stage in Trypanosoma cruzi.

Molecular Mechanisms of Host Cell Invasion by Trypanosoma cruzi

Enucleated L929 Cells Support Invasion, Differentiation, and Multiplication of Trypanosoma cruzi Parasites▿