NDLI: Identical, Independent, and Opposing Roles of ppGpp and DksA in Escherichia coli ▿

Content Provider	PubMed Central
Author	Magnusson, Lisa U. Gummesson, Bertil Joksimović, Predrag Farewell, Anne Nyström, Thomas
Copyright Year	2007
Abstract	The recent discovery that the protein DksA acts as a coregulator of genes controlled by ppGpp led us to investigate the similarities and differences between the relaxed phenotype of a ppGpp-deficient mutant and the phenotype of a strain lacking DksA. We demonstrate that the absence of DksA and ppGpp has similar effects on many of the observed phenotypes but that DksA and ppGpp also have independent and sometimes opposing roles in the cell. Specifically, we show that overexpression of DksA can compensate for the loss of ppGpp with respect to transcription of the promoters P uspA , P livJ , and P rrnBP1 as well as amino acid auxotrophy, cell-cell aggregation, motility, filamentation, and stationary phase morphology, suggesting that DksA can function without ppGpp in regulating gene expression. In addition, ppGpp and DksA have opposing effects on adhesion. In the course of our analysis, we also discovered new features of the relaxed mutant, namely, defects in cell-cell aggregation and motility.
Related Links	http://dx.doi.org/10.1128/jb.00330-07
Ending Page	5202
Page Count	10
Starting Page	5193
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	14
Volume Number	189
Language	English
Publisher	American Society for Microbiology
Publisher Date	2007-07-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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