NDLI: Kinetics of the Human Antibody Response against Salmonella enterica Serovars Enteritidis and Typhimurium Determined by Lipopolysaccharide Enzyme-Linked Immunosorbent Assay▿

Content Provider	PubMed Central
Author	Strid, Mette A. Dalby, Tine Mølbak, Kåre Krogfelt, Karen A.
Copyright Year	2007
Abstract	Two indirect enzyme-linked immunosorbent assays (ELISAs) were employed to measure levels of immunoglobulin G (IgG), IgM, and IgA antibodies against Salmonella in sera from 303 Danish patients diagnosed by fecal culture with either Salmonella enterica serovar Enteritidis or Salmonella enterica serovar Typhimurium infections. The ELISAs were based on serovar Enteritidis lipopolysaccharide (LPS) and serovar Typhimurium LPS. The antibody levels were assessed approximately 1, 3, 6, and 12 months after the onset of salmonellosis. Sera from 164 healthy blood donors were analyzed to establish cutoff values for each analysis. One month after the onset of symptoms, the sensitivities of the assays were 95% for patients recovering from a serovar Enteritidis infection and 89% for patients recovering from a serovar Typhimurium infection. Three months after the onset of symptoms, these values had decreased to 85% and 55%. At 6 months they were 62% and 40%, and at 12 months they were 40% and 16%, respectively. The specificities of the assays were 97% for the serovar Enteritidis LPS ELISA and 94% for the serovar Typhimurium LPS ELISA. The high values for both sensitivity and specificity make these two ELISAs useful for serodiagnoses of Salmonella infection shortly after the acute phase of the infection and of Salmonella-associated reactive arthritis, as well as for seroepidemiological studies. A mixed ELISA consisting of both antigens, i.e., serovar Enteritidis and serovar Typhimurium LPS, was developed as a diagnostic tool with very high values for both specificity and sensitivity.
Related Links	http://dx.doi.org/10.1128/cvi.00192-06
Ending Page	747
Page Count	7
Starting Page	741
File Format	PDF
ISSN	15566811
e-ISSN	1556679X
Journal	Clinical and Vaccine Immunology : CVI
Issue Number	6
Volume Number	14
Language	English
Publisher	American Society for Microbiology
Publisher Date	2007-06-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Immunology Immunology and Allergy Microbiology (medical) Clinical Biochemistry Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Clinical Biochemistry Immunology Microbiology (medical)

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