NDLI: Real-time monitoring of functional interactions between upstream and core promoter sequences in living cells of sea urchin embryos

Content Provider	PubMed Central
Author	Kobayashi, Akiko Watanabe, Youko Akasaka, Koji Kokubo, Tetsuro
Copyright Year	2007
Abstract	There are some functional compatibilities between upstream and core promoter sequences for transcriptional activation in yeast, Drosophila and mammalian cells. Here we examined whether similar compatibilities exist in sea urchin embryos, and if so, whether they are dynamically regulated during early development. Two reporter plasmids, each containing a test promoter conjugated to either CFP or YFP, were concurrently introduced into embryos, and their expression patterns were studied by fluorescence microscopy. The upstream sequence of the Hemicentrotus pulcherrimus (Hp) OtxE promoter drives the expression of its own core promoter and that of Strongylocentrotus purpuratus (Sp) Spec2a in different embryonic regions, especially at the late gastrula stage. Interestingly, when the four putative transcription factor binding sites of this upstream sequence were individually mutated, the resulting sequences directed different spatiotemporal expression from the same set of two core promoters, indicating that combinations of upstream factors may determine core promoter usage in sea urchin embryos. In addition, the insertion or deletion of consensus or nonconsensus TATA sequences changed the expression profile significantly, irrespective of whether the upstream sequence was intact or mutated. Thus, the TATA sequence may serve as a primary determinant for core promoter selection in these cells.
Related Links	http://dx.doi.org/10.1093/nar/gkm519
Starting Page	4882
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	14
Volume Number	35
Language	English
Publisher	Oxford University Press
Publisher Date	2007-07-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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Real-time monitoring of functional interactions between upstream and core promoter sequences in living cells of sea urchin embryos

Functional interaction between TATA and upstream CACGTG elements regulates the temporally specific expression of Otx mRNAs during early embryogenesis of the sea urchin, Hemicentrotus pulcherrimus

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Functional interaction between TATA and upstream CACGTG elements regulates the temporally specific expression of Otx mRNAs during early embryogenesis of the sea urchin, Hemicentrotus pulcherrimus

Real-time monitoring of functional interactions between upstream and core promoter sequences in living cells of sea urchin embryos

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Real-time monitoring of functional interactions between upstream and core promoter sequences in living cells of sea urchin embryos

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