NDLI: Glutamate receptor activation evokes calpain-mediated degradation of Sp3 and Sp4, the prominent Sp-family transcription factors in neurons

Content Provider	PubMed Central
Author	Mao, Xianrong Yang, Shao-hua Simpkins, James W. Barger, Steven W.
Abstract	Sp-family transcription factors (Sp1, Sp3 and Sp4) contain a zinc-finger domain that binds to DNA sequences rich in G-C/T. As assayed by RT-PCR analysis of mRNA, western blot analysis, immunofluorescence, and antibody-dependent “supershift” of DNA-binding assays, the prominent Sp-family factors in cerebral neurons were identified as Sp3 and Sp4. By contrast, glial cells were found to express Sp1 and Sp3. We previously showed that the pattern of G-C/T binding activity of Sp-family factors is rapidly and specifically altered by the calcium influx accompanying activation of glutamate receptors. Here, we demonstrate that Sp-factor activity is also lost after a cerebral ischemia/reperfusion injury in vivo. Consistent with its calcium-dependent nature, we found that glutamate’s effect on Sp-family factors could be blocked by inhibitors of calpains, neutral cysteine proteases activated by calcium. Purified calpain I cleaved Sp3 and Sp4 into products that retained G-C/T-binding activity, consistent with species observed in glutamate-treated neurons. These data provide details of an impact of glutamate-receptor activation on molecular events connected to gene expression.
Related Links	http://dx.doi.org/10.1111/j.1471-4159.2006.04297.x
Ending Page	1314
Page Count	15
Starting Page	1300
File Format	PDF
ISSN	00223042
e-ISSN	14714159
Journal	Journal of neurochemistry
Issue Number	5
Volume Number	100
Language	English
Publisher Date	2007-03-01
Access Restriction	Open
Subject Keyword	Biochemistry Cellular and Molecular Neuroscience Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biochemistry Cellular and Molecular Neuroscience

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