NDLI: RelA Is a Component of the Nutritional Stress Activation Pathway of the Bacillus subtilis Transcription Factor σB

Content Provider	PubMed Central
Author	Zhang, Shuyu Haldenwang, W. G.
Copyright Year	2003
Abstract	The general stress regulon of Bacillus subtilis is induced by the activation of the σB transcription factor. Activation of σB occurs when one of two phosphatases (RsbU and RsbP), each responding to a unique type of stress, actuates a positive regulator of σB by dephosphorylation. Nutritional stress triggers the RsbP phosphatase. The mechanism by which RsbP becomes active is unknown; however, its activation coincides with culture conditions that are likely to reduce the cell's levels of high-energy nucleotides. We now present evidence that RelA, a (p)ppGpp synthetase and the key enzyme of the stringent response, plays a role in nutritional stress activation of σB. An insertion mutation that disrupts relA blocks the activation of σB in response to PO4 or glucose limitation and inhibits the drop in ATP/GTP levels that normally accompanies σB induction under these conditions. In contrast, the activation of σB by physical stress (e.g., ethanol treatment) is not affected by the loss of RelA. RelA's role in σB activation appears to be distinct from its participation in the stringent response. Amino acid analogs which induce the stringent response and RelA-dependent (p)ppGpp synthesis do not trigger σB activity. In addition, neither a missense mutation in relA (relA240GE) nor a null mutation in rplK (rplK54), either of which is sufficient to inhibit the stringent response and RelA-dependent (p)ppGpp synthesis, fails to block σB activation by PO4 or glucose limitation.
Related Links	http://dx.doi.org/10.1128/JB.185.19.5714-5721.2003
Starting Page	5714
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	19
Volume Number	185
Language	English
Publisher	American Society for Microbiology
Publisher Date	2003-10-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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Contributions of ATP, GTP, and Redox State to Nutritional Stress Activation of the Bacillus subtilis σB Transcription Factor

ClpP Modulates the Activity of the Bacillus subtilis Stress Response Transcription Factor, σB ▿

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Catalytic Function of an α/β Hydrolase Is Required for Energy Stress Activation of the ςB Transcription Factor in Bacillus subtilis

General stress transcription factor sigmaB of Bacillus subtilis is a stable protein.

RelA Is a Component of the Nutritional Stress Activation Pathway of the Bacillus subtilis Transcription Factor σB

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RelA Is a Component of the Nutritional Stress Activation Pathway of the Bacillus subtilis Transcription Factor σB