NDLI: A gene from the cellulose synthase-like C family encodes a β-1,4 glucan synthase

Content Provider	PubMed Central
Author	Cocuron, Jean-christophe Olivier, Lerouxel Georgia, Drakakaki Alonso, Ana P. Liepman, Aaron H. Keegstra, Kenneth Raikhel, Natasha Wilkerson, Curtis G.
Copyright Year	2007
Abstract	Despite the central role of xyloglucan (XyG) in plant cell wall structure and function, important details of its biosynthesis are not understood. To identify the gene(s) responsible for synthesizing the β-1,4 glucan backbone of XyG, we exploited a property of nasturtium (Tropaeolum majus) seed development. During the last stages of nasturtium seed maturation, a large amount of XyG is deposited as a reserve polysaccharide. A cDNA library was produced from mRNA isolated during the deposition of XyG, and partial sequences of 10,000 cDNA clones were determined. A single member of the C subfamily from the large family of cellulose synthase-like (CSL) genes was found to be overrepresented in the cDNA library. Heterologous expression of this gene in the yeast Pichia pastoris resulted in the production of a β-1,4 glucan, confirming that the CSLC protein has glucan synthase activity. The Arabidopsis CSLC4 gene, which is the gene with the highest sequence similarity to the nasturtium CSL gene, is coordinately expressed with other genes involved in XyG biosynthesis. These and other observations provide a compelling case that the CSLC gene family encode proteins that synthesize the XyG backbone.
Related Links	http://dx.doi.org/10.1073/pnas.0703133104
Ending Page	8555
Page Count	6
Starting Page	8550
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	20
Volume Number	104
Language	English
Publisher	National Academy of Sciences
Publisher Date	2007-05-15
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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