NDLI: Efficient selection of recombinant adenoviruses by vectors that express beta-galactosidase.

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The vif gene is essential for efficient replication of caprine arthritis encephalitis virus in goat synovial membrane cells and affects the late steps of the virus replication cycle.

Binding and internalization of human papillomavirus type 33 virus-like particles by eukaryotic cells.

Regulation of transcription from the hepatitis B virus large surface antigen promoter by hepatocyte nuclear factor 3.

Dual human immunodeficiency virus type 1 infection and recombination in a dually exposed transfusion recipient. The Transfusion Safety Study Group.

The bovine leukemia virus encapsidation signal is discontinuous and extends into the 5' end of the gag gene.

Sequential isolation of proteoglycan synthesis mutants by using herpes simplex virus as a selective agent: evidence for a proteoglycan-independent virus entry pathway.

Brucella abortus conjugated with a gp120 or V3 loop peptide derived from human immunodeficiency virus (HIV) type 1 induces neutralizing anti-HIV antibodies, and the V3-B. abortus conjugate is effective even after CD4+ T-cell depletion.

Requirement of N-terminal amino acid residues of gp41 for human immunodeficiency virus type 1-mediated cell fusion.

Nuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutants.

Function of a unique sequence motif in the long terminal repeat of feline leukemia virus isolated from an unusual set of naturally occurring tumors.

Neutralizing monoclonal antibodies against human immunodeficiency virus type 2 gp120.

Functional and structural interactions between measles virus hemagglutinin and CD46.

The covalently closed duplex form of the hepadnavirus genome exists in situ as a heterogeneous population of viral minichromosomes.

Activation of a heterogeneous hepatitis B (HB) core and e antigen-specific CD4+ T-cell population during seroconversion to anti-HBe and anti-HBs in hepatitis B virus infection.

Mutations in the DNA-binding and dimerization domains of v-Rel are responsible for altered kappa B DNA-binding complexes in transformed cells.

Human papillomavirus type 31b late gene expression is regulated through protein kinase C-mediated changes in RNA processing.

Targeting foreign proteins to human immunodeficiency virus particles via fusion with Vpr and Vpx.

Reduced glycosylation of human cell lines increases susceptibility to CD4-independent infection by human immunodeficiency virus type 2 (LAV-2/B).

The spacer peptide between human immunodeficiency virus capsid and nucleocapsid proteins is essential for ordered assembly and viral infectivity.

Protein domains involved in both in vivo and in vitro interactions between human T-cell leukemia virus type I tax and CREB.

The envelope glycoprotein of an amphotropic murine retrovirus binds specifically to the cellular receptor/phosphate transporter of susceptible species.

The small envelope glycoprotein (GS) of equine arteritis virus folds into three distinct monomers and a disulfide-linked dimer.

Analysis of second-site revertants of a murine coronavirus nucleocapsid protein deletion mutant and construction of nucleocapsid protein mutants by targeted RNA recombination.

The short transcript of Leishmania RNA virus is generated by RNA cleavage.

Foot-and-mouth disease virus Lb proteinase can stimulate rhinovirus and enterovirus IRES-driven translation and cleave several proteins of cellular and viral origin.

Protection from La Crosse virus encephalitis with recombinant glycoproteins: role of neutralizing anti-G1 antibodies.

Cell surface proteoglycans are not essential for infection by pseudorabies virus.

Identification and characterization of a cDNA derived from multiple splicing that encodes envelope glycoprotein gp105 of human herpesvirus 6.

A TEF-1-independent mechanism for activation of the simian virus 40 (SV40) late promoter by mutant SV40 large T antigens.

Determination of human immunodeficiency virus RNA in plasma and cellular viral DNA genotypic zidovudine resistance and viral load during zidovudine-didanosine combination therapy.

Role of mannose-6-phosphate receptors in herpes simplex virus entry into cells and cell-to-cell transmission.

Inducible and conditional inhibition of human immunodeficiency virus proviral expression by vesicular stomatitis virus matrix protein.

Specific inhibition of granzyme B by parainfluenza virus type 3.

A maltose-binding protein/adeno-associated virus Rep68 fusion protein has DNA-RNA helicase and ATPase activities.

Comparative analyses of human immunodeficiency virus type 1 (HIV-1) and HIV-2 Vif mutants.

Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59.

Assembly of vaccinia virus: incorporation of p14 and p32 into the membrane of the intracellular mature virus.

The CAGT motif functions as an initiator element during early transcription of the baculovirus transregulator ie-1.

Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs.

Isolation of new oncogenic forms of the murine c-fms gene.

RNA-protein interactions directed by the 3' end of human rhinovirus genomic RNA.

The regulation of synthesis and properties of the protein product of open reading frame P of the herpes simplex virus 1 genome.

Epstein-Barr virus nuclear antigen 3C is a transcriptional regulator.

Tissue- and transformation-specific phosphotyrosyl proteins in v-erbB-transformed cells.

Nucleotide sequence of feline immunodeficiency virus: classification of Japanese isolates into two subtypes which are distinct from non-Japanese subtypes.

Overlapping initiator and TATA box functions in the basal core promoter of hepatitis B virus.

Interaction between the 5'-terminal cloverleaf and 3AB/3CDpro of poliovirus is essential for RNA replication.

Evidence that the potyvirus P1 proteinase functions in trans as an accessory factor for genome amplification.

Nascent human immunodeficiency virus type 1 reverse transcription occurs within an enveloped particle.

Cytopathogenicity of classical swine fever virus caused by defective interfering particles.

Assembly of the herpes simplex virus capsid: requirement for the carboxyl-terminal twenty-five amino acids of the proteins encoded by the UL26 and UL26.5 genes.

Enhancement of feline immunodeficiency virus infection after immunization with envelope glycoprotein subunit vaccines.

Growth of macrophage-tropic and primary human immunodeficiency virus type 1 (HIV-1) isolates in a unique CD4+ T-cell clone (PM1): failure to downregulate CD4 and to interfere with cell-line-tropic HIV-1.

Identification of regions in polyomavirus middle T and small t antigens important for association with protein phosphatase 2A.

Amino-terminal regions of polyomavirus middle T antigen are required for interactions with protein phosphatase 2A.

Growth kinetics of human cytomegalovirus are altered in monocyte-derived macrophages.

A subpopulation of normal B cells latently infected with Epstein-Barr virus resembles Burkitt lymphoma cells in expressing EBNA-1 but not EBNA-2 or LMP1.

Tissue-specific and ubiquitous factors binding next to the glucocorticoid receptor modulate transcription from the mouse mammary tumor virus promoter.

A molecular clasp in the human immunodeficiency virus (HIV) type 1 TM protein determines the anti-HIV activity of gp41 derivatives: implication for viral fusion.

Analysis of envelope sequence variants suggests multiple mechanisms of mother-to-child transmission of human immunodeficiency virus type 1.

E1 recognition sequences in the bovine papillomavirus type 1 origin of DNA replication: interaction between half sites of the inverted repeats.

Identification of an RNA binding region within the N-terminal third of the influenza A virus nucleoprotein.

Serotyping of primary human immunodeficiency virus type 1 isolates from diverse geographic locations by flow cytometry.

The Zif268 cellular transcription factor activates expression of the Epstein-Barr virus immediate-early BRLF1 promoter.

Rescue of human immunodeficiency virus type 1 matrix protein mutants by envelope glycoproteins with short cytoplasmic domains.

Retinoid activation of retinoic acid receptors but not of retinoid X receptors promotes cellular differentiation and replication of human cytomegalovirus in embryonal cells.

Selective depletion of stored calcium by thapsigargin blocks rotavirus maturation but not the cytopathic effect.

The rubella virus RNA binding activity of human calreticulin is localized to the N-terminal domain.

The D1 and D12 subunits are both essential for the transcription termination factor activity of vaccinia virus capping enzyme.

Nuclear localization and transcriptional activation activities of truncated versions of the immediate-early gene product of equine herpesvirus 1.

Characterization of bovine herpesvirus 1 UL49 homolog gene and product: bovine herpesvirus 1 UL49 homolog is dispensable for virus growth.

Isolation and characterization of a low-abundance splice variant from the human cytomegalovirus major immediate-early gene region.

Coxsackieviruses B1, B3, and B5 use decay accelerating factor as a receptor for cell attachment.

Domains upstream of the protease (PR) in human immunodeficiency virus type 1 Gag-Pol influence PR autoprocessing.

Novel integration sites at the distal 3' end of the c-myb locus in retrovirus-induced promonocytic leukemias.

The complement control protein homolog of herpesvirus saimiri regulates serum complement by inhibiting C3 convertase activity.

Hepatitis C virus nonstructural protein NS3 transforms NIH 3T3 cells.

Human parvovirus B19 can infect cynomolgus monkey marrow cells in tissue culture.

Isolation of Epstein-Barr virus-infected clones of the human T-cell line MT-2: use of recombinant viruses with a positive selection marker.

Human MxA protein inhibits tick-borne Thogoto virus but not Dhori virus.

Characterization of cytotoxic cells from reovirus-infected SCID mice: activated cells express natural killer- and lymphokine-activated killer-like activity but fail to clear infection.

Characterization of mouse parvovirus infection by in situ hybridization.

Efficient selection of recombinant adenoviruses by vectors that express beta-galactosidase.

The lef-3 gene of Autographa californica nuclear polyhedrosis virus encodes a single-stranded DNA-binding protein.

Increasing transduction efficiency of recombinant murine retrovirus vectors by initiation of endogenous reverse transcription: potential utility for genetic therapies.

Brain- and intestine-specific variants of reovirus serotype 3 strain dearing are selected during chronic infection of severe combined immunodeficient mice.

Human immunodeficiency virus type 1 preintegration complexes containing discontinuous plus strands are competent to integrate in vitro.

Inactivation of the retinoblastoma susceptibility protein is not sufficient for the transforming function of the conserved region 2-like domain of simian virus 40 large T antigen.

Role of the basic domain of human immunodeficiency virus type 1 matrix in macrophage infection.

Apoptosis as a cause of death in measles virus-infected cells.

Immunization with viruslike particles from cottontail rabbit papillomavirus (CRPV) can protect against experimental CRPV infection.

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Efficient selection of recombinant adenoviruses by vectors that express beta-galactosidase.

Content Provider	PubMed Central
Author	Schaack, J. Langer, S. Guo, X.
Abstract	Adenovirus serotype 5 vectors which contain the Excherichia coli beta-galactosidase gene driven by the cytomegalovirus immediate-early promoter as a screenable marker have been made and successfully used in the construction of recombinant adenoviruses. The beta-galactosidase gene has been introduced into viruses in which the E3 region is maintained or deleted and in which the cis-acting packaging sequence has been reiterated at the right end of the chromosome. A unique BstBI site has been introduced 3' of the beta-galactosidase gene. Cotransfection of BstBI-digested vector DNA and a plasmid containing the left end of the viral chromosome followed by staining with X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) results in clear plaques when overlap recombination has occurred and blue plaques when ligation of the viral arms has occurred within the host cell. The beta-galactosidase-expressing viruses grow to lower titers than do the parental viruses, leading to a relative growth advantage for viruses resulting from overlap recombination. Combined with color selection based on the beta-galactosidase gene, this system permits efficient production and selection of recombinant viruses after cotransfection of BstBI-digested viral DNA with a plasmid including left-end viral sequences and the gene of interest. The beta-galactosidase-expressing viral DNAs were used to construct viruses containing BstBI sites on either side of the cis-acting packaging element as a means of testing their utility.
Starting Page	3920
File Format	PDF
ISSN	10985514
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	6
Volume Number	69
Language	English
Publisher Date	1995-06-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

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