NDLI: Inactivation of nitric oxide by rat cerebellar slices

Content Provider	PubMed Central
Author	Hall, C. N. Garthwaite, J.
Copyright Year	2006
Abstract	Nitric oxide (NO) functions as an intercellular messenger throughout the brain. For this role to be performed efficiently, there must be a mechanism for neutralizing NO, but whether an active biological process exists, or whether NO is lost mainly through diffusion is unclear. To investigate this issue, rat cerebellar slices were exposed to constant levels of NO and the cGMP generated within the slice used as an indicator of NO concentrations therein. NO was about 1000-fold less potent in slices (EC50, 1 μm) than in separated cells from the same tissue (EC50, 1.6 nm), consistent with access of NO to the slice interior being greatly hindered by inactivation. Supporting this interpretation, immunohistochemical analysis indicated a marked concentration gradient of cGMP across the thickness of slices exposed to subsaturating NO concentrations, signifying a marked NO gradient. Several known NO-degrading processes, including reaction with lipid peroxyl radicals, erythrocytes and superoxide ions, were eliminated as contributing factors, indicating a novel mechanism. A diffusion–inactivation model was used to estimate the kinetics of NO consumption by the slices. The best fits to experimental data indicated a Michaelis-Menten-type reaction having a V max of 1–2 μm s−1 and a K m of around 10 nm. The rates predict that inactivation would impose a very short half-life (<10 ms) on NO in physiological concentrations (up to 10 nm) and that it would play an important role in shaping the NO concentration profiles when it is synthesized by multiple nearby sites.
Related Links	http://dx.doi.org/10.1113/jphysiol.2006.118380
Ending Page	567
Page Count	19
Starting Page	549
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 2
Volume Number	577
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2006-12-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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