NDLI: Engineered serine protease inhibitor prevents furin-catalyzed activation of the fusion glycoprotein and production of infectious measles virus.

Please wait, while we are loading the content...

Year: 2016, Volume: 90

Year: 2015, Volume: 90

Year: 2015, Volume: 89

Year: 2014, Volume: 89

Year: 2014, Volume: 88

Year: 2013, Volume: 87

Year: 2012, Volume: 86

Year: 2011, Volume: 85

Year: 2010, Volume: 84

Year: 2009, Volume: 83

Year: 2008, Volume: 82

Year: 2007, Volume: 81

Year: 2006, Volume: 80

Year: 2005, Volume: 79

Year: 2004, Volume: 78

Year: 2003, Volume: 77

Year: 2002, Volume: 76

Year: 2001, Volume: 75

Year: 2000, Volume: 74

Year: 1999, Volume: 73

Year: 1998, Volume: 72

Year: 1997, Volume: 71

Year: 1996, Volume: 70

Year: 1995, Volume: 69

Year: 1995, Volume: 69, Issue: 12

Year: 1995, Volume: 69, Issue: 11

Year: 1995, Volume: 69, Issue: 10

Year: 1995, Volume: 69, Issue: 9

Year: 1995, Volume: 69, Issue: 8

Year: 1995, Volume: 69, Issue: 7

Year: 1995, Volume: 69, Issue: 6

Year: 1995, Volume: 69, Issue: 5

Reverse genetics system for generation of an influenza A virus mutant containing a deletion of the carboxyl-terminal residue of M2 protein.

Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication.

Cross-protective immune responses induced in rhesus macaques by immunization with attenuated macrophage-tropic simian immunodeficiency virus.

Oligomerization of the hydrophobic heptad repeat of gp41.

Defining the level of human immunodeficiency virus type 1 (HIV-1) protease activity required for HIV-1 particle maturation and infectivity.

The p6gag domain of human immunodeficiency virus type 1 is sufficient for the incorporation of Vpr into heterologous viral particles.

Molecular and functional interactions of transcription factor USF with the long terminal repeat of human immunodeficiency virus type 1.

Preferential recognition of hepatitis B nucleocapsid antigens by Th1 or Th2 cells is epitope and major histocompatibility complex dependent.

Regulatory function of the equine herpesvirus 1 ICP27 gene product.

Constitutive activation of a variant of the env-mpl oncogene product by disulfide-linked homodimerization.

Adaptation of human immunodeficiency virus type 1 to cells expressing a binding-deficient CD4 mutant (lysine 46 to aspartic acid).

Cloning, sequencing, and functional characterization of the two subunits of the pseudorabies virus DNA polymerase holoenzyme: evidence for specificity of interaction.

Interaction of eukaryotic initiation factor eIF-4B with a picornavirus internal translation initiation site.

Maturation of giardiavirus capsid protein involves posttranslational proteolytic processing by a cysteine protease.

Characterization and modification of the carboxy-terminal sequences of bluetongue virus type 10 NS1 protein in relation to tubule formation and location of an antigenic epitope in the vicinity of the carboxy terminus of the protein.

Zinc-binding and protein-protein interactions mediated by the polyomavirus large T antigen zinc finger.

Human adenovirus serotypes 3 and 5 bind to two different cellular receptors via the fiber head domain.

Localization of antigenic sites of the S glycoprotein of feline infectious peritonitis virus involved in neutralization and antibody-dependent enhancement.

Clonal expansion of human T-cell leukemia virus type I-infected cells in asymptomatic and symptomatic carriers without malignancy.

Size of genetic bottlenecks leading to virus fitness loss is determined by mean initial population fitness.

Analysis of the promoter and cis-acting elements regulating expression of herpes simplex virus type 2 latency-associated transcripts.

Replication in vitro and in vivo of an equine infectious anemia virus mutant deficient in dUTPase activity.

Two overlapping transcription units which extend across the L-S junction of herpes simplex virus type 1.

Transcriptional activity of core binding factor-alpha (AML1) and beta subunits on murine leukemia virus enhancer cores.

Repression in vitro, by human adenovirus E1A protein domains, of basal or Tat-activated transcription of the human immunodeficiency virus type 1 long terminal repeat.

Differential transcription of baculovirus late and very late promoters: fractionation of nuclear extracts by phosphocellulose chromatography.

Evolution of subgroup A respiratory syncytial virus: evidence for progressive accumulation of amino acid changes in the attachment protein.

In vitro packaging of individual genomic segments of bacteriophage phi 6 RNA: serial dependence relationships.

The Rev protein of human immunodeficiency virus type 1 counteracts the effect of an AU-rich negative element in the human papillomavirus type 1 late 3' untranslated region.

Replication and amplification of novel vesicular stomatitis virus minigenomes encoding viral structural proteins.

Poliovirus infection enhances the formation of two ribonucleoprotein complexes at the 3' end of viral negative-strand RNA.

The N-terminal region of the adenovirus type 5 E1A proteins can repress expression of cellular genes via two distinct but overlapping domains.

Stimulation of NF-kappa B-mediated transcription by mutant derivatives of the latent membrane protein of Epstein-Barr virus.

Establishment of a stable, inducible form of human immunodeficiency virus type 1 DNA in quiescent CD4 lymphocytes in vitro.

Integration of human papillomavirus type 16 into the human genome correlates with a selective growth advantage of cells.

Replication of Epstein-Barr virus oriLyt: lack of a dedicated virally encoded origin-binding protein and dependence on Zta in cotransfection assays.

Molecular cloning and characterization of a cellular protein that interacts with the human immunodeficiency virus type 1 Tat transactivator and encodes a strong transcriptional activation domain.

In vitro interaction of the human immunodeficiency virus type 1 Tat transactivator and the general transcription factor TFIIB with the cellular protein TAP.

Detection and intracellular localization of equine herpesvirus 1 IR1 and IR2 gene products by using monoclonal antibodies.

An avirulent ICP34.5 deletion mutant of herpes simplex virus type 1 is capable of in vivo spontaneous reactivation.

Repression of the herpes simplex virus 1 alpha 4 gene by its gene product (ICP4) within the context of the viral genome is conditioned by the distance and stereoaxial alignment of the ICP4 DNA binding site relative to the TATA box.

Control of baculovirus gp64-induced syncytium formation by membrane lipid composition.

Episomal amplification or chromosomal integration of the viral genome: alternative pathways in hamster polyomavirus-induced lymphomas.

Mutations in the epsilon sequences of human hepatitis B virus affect both RNA encapsidation and reverse transcription.

Analysis of genomic sequences of 95 papillomavirus types: uniting typing, phylogeny, and taxonomy.

Herpes simplex virus type 1 DNA replication is specifically required for high-frequency homologous recombination between repeated sequences.

Reduced replication of human immunodeficiency virus type 1 mutants that use reverse transcription primers other than the natural tRNA(3Lys).

Transcriptional trans activation by human immunodeficiency virus type 1 Tat requires specific coactivators that are not basal factors.

Epstein-Barr virus nuclear protein 3C modulates transcription through interaction with the sequence-specific DNA-binding protein J kappa.

Macaques immunized with HLA-DR are protected from challenge with simian immunodeficiency virus.

Effects of translation initiation factor eIF-5A on the functioning of human T-cell leukemia virus type I Rex and human immunodeficiency virus Rev inhibited trans dominantly by a Rex mutant deficient in RNA binding.

Functional analysis of amino acid residues encompassing and surrounding two neighboring H-2Db-restricted cytotoxic T-lymphocyte epitopes in simian virus 40 tumor antigen.

Structure and role of the terminal repeats of Epstein-Barr virus in processing and packaging of virion DNA.

Membrane vesiculation function and exocytosis of wild-type and mutant matrix proteins of vesicular stomatitis virus.

Immediate-early transcription from the channel catfish virus genome: characterization of two immediate-early transcripts.

Change in tropism upon immune escape by human immunodeficiency virus.

Theiler's virus as a vector for foreign gene delivery.

Sequence comparison of porcine respiratory coronavirus isolates reveals heterogeneity in the S, 3, and 3-1 genes.

The E5 oncoprotein of human papillomavirus type 16 inhibits the acidification of endosomes in human keratinocytes.

Attenuated Mengo virus: a new vector for live recombinant vaccines.

Early infection of the central nervous system by the GDVII and DA strains of Theiler's virus.

Bovine papillomavirus E1 protein can, by itself, efficiently drive multiple rounds of DNA synthesis in vitro.

Engineered serine protease inhibitor prevents furin-catalyzed activation of the fusion glycoprotein and production of infectious measles virus.

5' termini of Pichinde arenavirus S RNAs and mRNAs contain nontemplated nucleotides.

Integration is required for productive infection of monocyte-derived macrophages by human immunodeficiency virus type 1.

Sphingolipid-dependent fusion of Semliki Forest virus with cholesterol-containing liposomes requires both the 3-hydroxyl group and the double bond of the sphingolipid backbone.

A replication-competent, endogenous retrovirus from an aged DBA/2 mouse contains the complete env from Emv-3 and a novel gag partially related to AKT-8.

Myeloid depression follows infection of susceptible newborn mice with the parvovirus minute virus of mice (strain i).

Different roles of bases within the integration signal sequence of human immunodeficiency virus type 1 in vitro.

Echovirus 1 interaction with the isolated VLA-2 I domain.

Varicella-zoster virus gene 63 encodes an immediate-early protein that is abundantly expressed during latency.

Year: 1995, Volume: 69, Issue: 4

Year: 1995, Volume: 69, Issue: 3

Year: 1995, Volume: 69, Issue: 2

Year: 1995, Volume: 69, Issue: 1

Year: 1994, Volume: 68

Year: 1993, Volume: 67

Year: 1992, Volume: 66

Year: 1991, Volume: 65

Year: 1990, Volume: 64

Year: 1989, Volume: 63

Year: 1988, Volume: 62

Year: 1987, Volume: 61

Year: 1986, Volume: 60

Year: 1986, Volume: 59

Year: 1986, Volume: 58

Year: 1986, Volume: 57

Year: 1985, Volume: 56

Year: 1985, Volume: 55

Year: 1985, Volume: 54

Year: 1985, Volume: 53

Year: 1984, Volume: 52

Year: 1984, Volume: 51

Year: 1984, Volume: 50

Year: 1984, Volume: 49

Year: 1983, Volume: 48

Year: 1983, Volume: 47

Year: 1983, Volume: 46

Year: 1983, Volume: 45

Year: 1982, Volume: 44

Year: 1982, Volume: 43

Year: 1982, Volume: 42

Year: 1982, Volume: 41

Year: 1981, Volume: 40

Year: 1981, Volume: 39

Year: 1981, Volume: 38

Year: 1981, Volume: 37

Year: 1980, Volume: 36

Year: 1980, Volume: 35

Year: 1980, Volume: 34

Year: 1980, Volume: 33

Year: 1979, Volume: 32

Year: 1979, Volume: 31

Year: 1979, Volume: 30

Year: 1979, Volume: 29

Year: 1978, Volume: 28

Year: 1978, Volume: 27

Year: 1978, Volume: 26

Year: 1978, Volume: 25

Year: 1977, Volume: 24

Year: 1977, Volume: 23

Year: 1977, Volume: 22

Year: 1977, Volume: 21

Year: 1976, Volume: 20

Year: 1976, Volume: 19

Year: 1976, Volume: 18

Year: 1976, Volume: 17

Year: 1975, Volume: 16

Year: 1975, Volume: 15

Year: 1974, Volume: 14

Year: 1974, Volume: 13

Year: 1973, Volume: 12

Year: 1973, Volume: 11

Year: 1972, Volume: 10

Year: 1972, Volume: 9

Year: 1971, Volume: 8

Year: 1971, Volume: 7

Year: 1970, Volume: 6

Year: 1970, Volume: 5

Year: 1969, Volume: 4

Year: 1969, Volume: 3

Year: 1968, Volume: 2

Year: 1967, Volume: 1

Engineered serine protease inhibitor prevents furin-catalyzed activation of the fusion glycoprotein and production of infectious measles virus.

Content Provider	PubMed Central
Author	Watanabe, M. Hirano, A. Stenglein, S. Nelson, J. Thomas, G. Wong, T. C.
Abstract	We have identified the major cellular endoprotease that activates the fusion (F) glycoprotein of measles virus (MV) and have engineered a serine protease inhibitor (serpin) to target the endoprotease and inhibit the production of infectious MV. The F-protein precursor of MV was not cleaved efficiently into the mature F protein in human colon carcinoma cells lacking functional furin, indicating that furin is the major enzyme responsible for activation of the MV F protein. A human serpin alpha 1-antitrypsin variant was engineered to specifically inhibit furin. When expressed from a recombinant vaccinia virus in primate cells infected by MV, the engineered serpin (alpha 1-PDX) specifically inhibited furin-catalyzed cleavage of the F-protein precursor without affecting synthesis of other MV proteins. We generated human glioma cells stably expressing alpha 1-PDX. MV infection in these cells did not result in syncytia. The infected cells produced all the MV proteins, but the F-protein precursor remained largely uncleaved. This did not prevent virus assembly. However, the released virions contained inactive F-protein precursor rather than mature F protein, and infectious-virus titers were reduced by 3 to 4 orders of magnitude. These results show that a mature F protein is not required for the assembly of MV but is crucial for virus infectivity. The engineered serpin may offer a novel molecular antiviral approach against MV.
Starting Page	3206
File Format	PDF
ISSN	10985514
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	5
Volume Number	69
Language	English
Publisher Date	1995-05-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in