NDLI: High-speed mapping of synaptic connectivity using photostimulation in Channelrhodopsin-2 transgenic mice

Content Provider	PubMed Central
Author	Wang, H. Peca, J. Matsuzaki, M. Matsuzaki, K. Noguchi, J. Qiu, L. Wang, D. Zhang, F. Boyden, E. Deisseroth, K. Kasai, H. Hall, W. C. Feng, G. Augustine, G. J.
Copyright Year	2007
Abstract	To permit rapid optical control of brain activity, we have engineered multiple lines of transgenic mice that express the light-activated cation channel Channelrhodopsin-2 (ChR2) in subsets of neurons. Illumination of ChR2-positive neurons in brain slices produced photocurrents that generated action potentials within milliseconds and with precisely timed latencies. The number of light-evoked action potentials could be controlled by varying either the amplitude or duration of illumination. Furthermore, the frequency of light-evoked action potentials could be precisely controlled up to 30 Hz. Photostimulation also could evoke synaptic transmission between neurons, and, by scanning with a small laser light spot, we were able to map the spatial distribution of synaptic circuits connecting neurons within living cerebral cortex. We conclude that ChR2 is a genetically based photostimulation technology that permits analysis of neural circuits with high spatial and temporal resolution in transgenic mammals.
Related Links	http://dx.doi.org/10.1073/pnas.0700384104
Ending Page	8148
Page Count	6
Starting Page	8143
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	19
Volume Number	104
Language	English
Publisher	National Academy of Sciences
Publisher Date	2007-05-08
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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