NDLI: The symbiosis regulator RscS controls the syp gene locus, biofilm formation and symbiotic aggregation by Vibrio fischeri

Content Provider	PubMed Central
Author	Yip, Emily S. Kati, Geszvain Deloney-marino, Cindy R. Visick, Karen L.
Abstract	Successful colonization of a eukaryotic host by a microbe involves complex microbe-microbe and microbe-host interactions. Previously, we identified in Vibrio fischeri a putative sensor kinase, RscS, required for initiating symbiotic colonization of its squid host Euprymna scolopes. Here, we analyzed the role of rscS by isolating an allele, rscS1, with increased activity. Multi-copy rscS1 activated transcription of genes within the recently identified symbiosis polysaccharide (syp) cluster. Wild-type cells carrying rscS1 induced aggregation phenotypes in culture, including the formation of pellicles and wrinkled colonies, in a syp-dependent manner. Colonies formed by rscS1-expressing cells produced a matrix not found in control colonies and largely lost in an rscS1-expressing sypN mutant. Finally, multi-copy rscS1 provided a colonization advantage over control cells and substantially enhanced the ability of wild-type cells to aggregate on the surface of the symbiotic organ of E. scolopes; this latter phenotype similarly depended upon an intact syp locus. These results suggest that transcription induced by RscS-mediated signal transduction plays a key role in colonization at the aggregation stage by modifying the cell surface and increasing the ability of the cells to adhere to one another and/or to squid-secreted mucus.
Related Links	http://dx.doi.org/10.1111/j.1365-2958.2006.05475.x
Ending Page	1600
Page Count	15
Starting Page	1586
File Format	PDF
ISSN	0950382X
e-ISSN	13652958
Journal	Molecular microbiology
Issue Number	6
Volume Number	62
Language	English
Publisher Date	2006-12-01
Access Restriction	Open
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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RscS Functions Upstream of SypG To Control the syp Locus and Biofilm Formation in Vibrio fischeri ▿

The response regulator SypE controls biofilm formation and colonization through phosphorylation of the syp-encoded regulator SypA in Vibrio fischeri

Roles of the Structural Symbiosis Polysaccharide (syp) Genes in Host Colonization, Biofilm Formation, and Polysaccharide Biosynthesis in Vibrio fischeri

The syp Enhancer Sequence Plays a Key Role in Transcriptional Activation by the σ54-Dependent Response Regulator SypG and in Biofilm Formation and Host Colonization by Vibrio fischeri

The Hybrid Sensor Kinase RscS Integrates Positive and Negative Signals To Modulate Biofilm Formation in Vibrio fischeri ▿

Sensor Kinase RscS Induces the Production of Antigenically Distinct Outer Membrane Vesicles That Depend on the Symbiosis Polysaccharide Locus in Vibrio fischeri

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Inactivation of a novel response regulator is necessary for biofilm formation and host colonization by Vibrio fischeri

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The symbiosis regulator RscS controls the syp gene locus, biofilm formation and symbiotic aggregation by Vibrio fischeri

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