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| Content Provider | PubMed Central |
|---|---|
| Author | Abdurashidova, Gulnara Danailov, Miltcho B. Ochem, Alexander Triolo, Gianluca Vera, Djeliova Radulescu, Sorina Alessandro, Vindigni Riva, Silvano Falaschi, Arturo |
| Copyright Year | 2003 |
| Abstract | The proteins bound in vivo at the human lamin B2 DNA replication origin and their precise sites of binding were investigated along the cell cycle utilizing two novel procedures based on immunoprecipitation following UV irradiation with a pulsed laser light source. In G1, the pre-replicative complex contains CDC6, MCM3, ORC1 and ORC2 proteins; of these, the post-replicative complex in S phase contains only ORC2; in M phase none of them are bound. The precise nucleotide of binding was identified for the two ORC and the CDC6 proteins near the start sites for leading-strand synthesis; the transition from the pre- to the post-replicative complex is accompanied by a 17 bp displacement of the ORC2 protein towards the start site. |
| Related Links | http://dx.doi.org/10.1093/emboj/cdg404 |
| Ending Page | 4303 |
| Page Count | 10 |
| Starting Page | 4294 |
| File Format | |
| ISSN | 14602075 |
| e-ISSN | 14602075 |
| Journal | The EMBO Journal |
| Issue Number | 16 |
| Volume Number | 22 |
| Language | English |
| Publisher | Oxford University Press |
| Publisher Date | 2003-08-15 |
| Access Restriction | Open |
| Rights Holder | Oxford University Press |
| Subject Keyword | Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience Immunology and Microbiology Medicine Molecular Biology |
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