NDLI: Detection of multiple viral DNA species in synovial tissue and fluid of patients with early arthritis

Content Provider	PubMed Central
Author	Stahl, H. Hubner, B. Seidl, B. Liebert, U. Heijden, I. M. Van Der Wilbrink, B. Kraan, M. Emmrich, F. Tak, P.
Abstract	OBJECTIVE—Viruses have a role in the pathogenesis of various forms of arthritis. This study aimed at determining whether viral DNA can be detected in joint samples in the early stages of idiopathic arthritides. METHODS—Synovial fluid (SF) and synovial tissue (ST) samples were obtained from 73 patients, with undifferentiated arthritis (n=22), rheumatoid arthritis (n=13), spondyloarthropathy (n=17), crystal arthropathy (n=8), osteoarthritis (n=7), septic arthritis (n=5), and trauma (n=1). The presence of viral DNA was investigated by polymerase chain reaction analysis. RESULTS—Cytomegalovirus was present in 25 patients, parvovirus B19 in 15 patients, Epstein-Barr virus in 12 patients, and herpes simplex virus in 16 patients (in ST, SF, or both), respectively. The joint samples were negative for viral DNA from adenovirus and varicella-zoster virus. In ST, eight patients were double positive for parvovirus B19 and another viral DNA, with herpes simplex virus being the most prevalent. Seven patients were double positive for other viruses (cytomegalovirus, herpes simplex virus, Epstein-Barr virus). In SF, four patients were double or triple positive for viral DNA. Paired samples were available in 56 patients. In these, viral DNA was detected in 37 patients in ST, as compared with 19 in SF. CONCLUSION—These data show that one or more viruses can be detected in the synovial specimens of patients with early arthritis, irrespective of the clinical diagnosis. This observation might be explained by migration of inflammatory cells harbouring viral DNA into the inflamed joints.
Related Links	http://dx.doi.org/10.1136/ard.59.5.342
Ending Page	346
Page Count	5
Starting Page	342
File Format	PDF
ISSN	00034967
e-ISSN	14682060
Journal	Annals of the Rheumatic Diseases
Issue Number	5
Volume Number	59
Language	English
Publisher Date	2000-05-01
Access Restriction	Open
Subject Keyword	Immunology Biochemistry, Genetics and Molecular Biology(all) Immunology and Allergy Rheumatology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Biochemistry, Genetics and Molecular Biology Immunology Rheumatology

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