NDLI: Novel insights into the genetics, biochemistry, and immunocytochemistry of the 30-kilodalton major extracellular protein of Mycobacterium tuberculosis.

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Transient and persistent experimental infection of nonhuman primates with Helicobacter pylori: implications for human disease.

Plasmodium falciparum stimuli for human gammadelta T cells are related to phosphorylated antigens of mycobacteria.

Capsular polysaccharide of Cryptococcus neoformans induces proinflammatory cytokine release by human neutrophils.

The major surface protein complex of Treponema denticola depolarizes and induces ion channels in HeLa cell membranes.

Identification of a transferrin-binding protein from Borrelia burgdorferi.

Epidermal growth factor-binding protein in mycobacterium avium and mycobacterium tuberculosis: a possible role in the mechanism of infection.

The Bcg/Ity/Lsh locus: genetic transfer of resistance to infections in C57BL/6J mice transgenic for the Nramp1 Gly169 allele.

Specific induction of fibronectin binding activity by hemoglobin in Candida albicans grown in defined media.

Induction of an extracellular esterase from Candida albicans and some of its properties.

Role of gamma interferon in a neonatal mouse model of group B streptococcal disease.

Miller-Fisher syndrome associated with Campylobacter jejuni bearing lipopolysaccharide molecules that mimic human ganglioside GD3.

Antibody-independent effector mechanisms in resistance to the intestinal nematode parasite Trichuris muris.

Immunity against Yersinia enterocolitica by vaccination with Yersinia HSP60 immunostimulating complexes or Yersinia HSP60 plus interleukin-12.

Th1-like antifilarial immune responses predominate in antigen-negative persons.

Mechanism of membrane damage by El Tor hemolysin of Vibrio cholerae O1.

Selective killing of human monocytes and cytokine release provoked by sphingomyelinase (beta-toxin) of Staphylococcus aureus.

Identification and characterization of the Yersinia enterocolitica gsrA gene, which protectively responds to intracellular stress induced by macrophage phagocytosis and to extracellular environmental stress.

Invasion of epithelial cells by Actinobacillus actinomycetemcomitans: a dynamic, multistep process.

Invasion of primary nasopharyngeal epithelial cells by Neisseria meningitidis is controlled by phase variation of multiple surface antigens.

Mitogenic activities of amino acid substitution mutants of staphylococcal enterotoxin B in human and mouse lymphocyte cultures.

Cytokine production by human epithelial and endothelial cells following exposure to oral viridans streptococci involves lectin interactions between bacteria and cell surface receptors.

Iron uptake and iron-repressible polypeptides in Yersinia pestis.

Identification of surface-exposed B-cell epitopes on high molecular-weight adhesion proteins of nontypeable Haemophilus influenzae.

Novel insights into the genetics, biochemistry, and immunocytochemistry of the 30-kilodalton major extracellular protein of Mycobacterium tuberculosis.

Localization of a yeast-phase-specific gene product to the cell wall in Histoplasma capsulatum.

Engineered deltaguaB-A deltavirG Shigella flexneri 2a strain CVD 1205: construction, safety, immunogenicity, and potential efficacy as a mucosal vaccine.

Induction of cytokine granulocyte-macrophage colony-stimulating factor and chemokine macrophage inflammatory protein 2 mRNAs in macrophages by Legionella pneumophila or Salmonella typhimurium attachment requires different ligand-receptor systems.

Experimental immunization of rats with a Streptococcus mutans 59-kilodalton glucan-binding protein protects against dental caries.

Counterprotective effect of erythrocytes in experimental bacterial peritonitis is due to scavenging of nitric oxide and reactive oxygen intermediates.

Escherichia coli hemolysin mutants with altered target cell specificity.

Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii.

Intermedilysin, a novel cytotoxin specific for human cells secreted by Streptococcus intermedius UNS46 isolated from a human liver abscess.

Purification and characterization of an extracellular secretogenic non-membrane-damaging cytotoxin produced by clinical strains of Vibrio cholerae non-O1.

Brucella abortus as a potential vaccine candidate: induction of interleukin-12 secretion and enhanced B7.1 and B7.2 and intercellular adhesion molecule 1 surface expression in elutriated human monocytes stimulated by heat-inactivated B. abortus.

Escherichia coli cellulitis in broiler chickens: clonal relationships among strains and analysis of virulence-associated factors of isolates from diseased birds.

Peroxynitrite contributes to the candidacidal activity of nitric oxide-producing macrophages.

Cloning of a DNA fragment encoding a heme-repressible hemoglobin-binding outer membrane protein from Haemophilus influenzae.

Influence of agr on fibrinogen binding in Staphylococcus aureus Newman.

Neurotoxicity of glia activated by gram-positive bacterial products depends on nitric oxide production.

Characterization of elastase-deficient clinical isolates of Pseudomonas aeruginosa.

Cloning, sequencing, expression, and protective capacity of the oma87 gene encoding the Pasteurella multocida 87-kilodalton outer membrane antigen.

Immune response in mice following immunization with DNA encoding fragment C of tetanus toxin.

Target organs of infection in guinea pigs with acquired congenital syphilis.

Outer surface lipoproteins of Borrelia burgdorferi activate vascular endothelium in vitro.

Induction of cytokine gene expression by listeriolysin O and roles of macrophages and NK cells.

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Pathogenicity island evaluation in Escherichia coli K1 by crossing with laboratory strain K-12.

Involvement of reactive oxygen intermediates in tumor necrosis factor alpha-dependent bacteriostasis of Mycobacterium avium.

Interleukin-6 is required for a protective immune response to systemic Escherichia coli infection.

Roles for tumor necrosis factor alpha and nitric oxide in resistance of rat alveolar macrophages to Legionella pneumophila.

Characterization of echinocandin-resistant mutants of Candida albicans: genetic, biochemical, and virulence studies.

Role of Pseudomonas aeruginosa lipase in inflammatory mediator release from human inflammatory effector cells (platelets, granulocytes, and monocytes.

Biochemical and molecular analysis of phospholipase C and phospholipase D activity in mycobacteria.

Proteins of 30 and 36 kilodaltons, membrane constituents of the Staphylococcus aureus L form, induce production of tumor necrosis factor alpha and activate the human immunodeficiency virus type 1 long terminal repeat.

Evidence for a predominant proinflammatory conjunctival cytokine response in individuals with trachoma.

Increased susceptibility to primary infection with Listeria monocytogenes in germfree mice may be due to lack of accumulation of L-selectin+ CD44+ T cells in sites of inflammation.

Minimal requirements for murine resistance to infection with Francisella tularensis LVS.

Translocation of Shiga toxin across polarized intestinal cells in tissue culture.

Regulated expression of Clostridium perfringens enterotoxin in naturally cpe-negative type A, B, and C isolates of C. perfringens.

Protective immune responses to apical membrane antigen 1 of Plasmodium chabaudi involve recognition of strain-specific epitopes.

Influence of lipoteichoic acid structure on recognition by the macrophage scavenger receptor.

The Cu,Zn superoxide dismutases of Aspergillus flavus, Aspergillus niger, Aspergillus nidulans, and Aspergillus terreus: purification and biochemical comparison with the Aspergillus fumigatus Cu,Zn superoxide dismutase.

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Role of nitric oxide in host defense against an extracellular, metazoan parasite, Brugia malayi.

Characterization of the murine antibody response to peptides representing the variable domains of the major outer membrane protein of Chlamydia pneumoniae.

Distinct characteristics of initiation of the classical and alternative complement pathways by Candida albicans.

Relative significance of mannose-sensitive hemagglutinin and toxin-coregulated pili in colonization of infant mice by Vibrio cholerae El Tor.

Functional characterization of a sialyltransferase-deficient mutant of Neisseria gonorrhoeae.

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Sequence of the toxic shock syndrome toxin gene (tstH) borne by strains of Staphylococcus aureus isolated from patients with Kawasaki syndrome.

Stimulation of the ceramide pathway partially mimics lipopolysaccharide-induced responses in murine peritoneal macrophages.

Characterization of PepB, a group B streptococcal oligopeptidase.

Interleukin-8 gene expression in Staphylococcus aureus-infected endothelial cells.

Purification and characterization of the staphylococcal slime-associated antigen and its occurrence among Staphylococcus epidermis clinical isolates.

Adhesion of K88ab to guinea pig erythrocytes: effect on membrane enzyme activities.

Pathogenicity of Mycoplasma fermentans and Mycoplasma penetrans in experimentally infected chicken embryos.

Ingestion of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli by human peritoneal mesothelial cells.

Granulocyte-macrophage colony-stimulating factor: involvement in control of Trypanosoma cruzi infection in mice.

Tumor necrosis factor primes neutrophils to kill Staphylococcus aureus by an oxygen-dependent mechanism and Plasmodium falciparum by an oxygen-independent mechanism.

Structural similarities among malaria toxins insulin second messengers, and bacterial endotoxin.

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Novel insights into the genetics, biochemistry, and immunocytochemistry of the 30-kilodalton major extracellular protein of Mycobacterium tuberculosis.

Content Provider	PubMed Central
Author	Harth, G. Lee, B. Y. Wang, J. Clemens, D. L. Horwitz, M. A.
Abstract	The 30/32-kDa complex of major secretory proteins are among the most important and intensively studied proteins of Mycobacterium tuberculosis. The proteins have been demonstrated to be immunoprotective and to play a central role in the physiology of the mycobacterium. In this study, we present a series of novel insights into this key protein complex arising out of a combination of genetic, biochemical, and immunocytochemical analyses. Our genetic analyses (i) indicate that the genes are arranged as separate transcription units, (ii) demonstrate that the mature 30-kDa protein of M. tuberculosis differs from the corresponding 30-kDa proteins of two strains of Mycobacterium bovis BCG by only 1 and 5 amino acids, (iii) suggest that expression of the proteins is regulated at the transcriptional level, and (iv) map the transcriptional start site of the 30-kDa protein gene. Our biochemical analyses provide evidence that (i) the 30-kDa protein and the two 32-kDa proteins (i.e., 32A and 32B) are secreted at a ratio of approximately 3:2:1, respectively, (ii) the proteins exist as monomers, (iii) the proteins are not posttranslationally modified by the addition of carbohydrates and lipids, (iv) the 30-kDa and 32A proteins contain one disulfide bridge, and (v) high-level expression and leader peptide processing are achievable in Escherichia coli. Our immunocytochemical analyses demonstrate that the 30/32-kDa complex is expressed in human monocytes and that the proteins are localized to the phagosomal space and the mycobacterial cell wall. These analyses fill important gaps in our knowledge of this critical protein complex of M. tuberculosis and, at the same time, raise new and fundamental questions regarding regulatory mechanisms that control coordinate expression of the proteins at a fixed ratio.
Starting Page	3038
File Format	PDF
ISSN	10985522
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	8
Volume Number	64
Language	English
Publisher Date	1996-08-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

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