NDLI: Variation in antigenicity and infectivity of derivatives of Borrelia burgdorferi, strain B31, maintained in the natural, zoonotic cycle compared with maintenance in culture.

Content Provider	PubMed Central
Author	Golde, W. T. Dolan, M. C.
Abstract	The original isolate of Borrelia burgdorferi, strain B31, can be maintained in vitro indefinitely. A number of studies have demonstrated that there are recognizable changes in the genetic composition of the spirochete after more than 60 passages. We have maintained B31 in the natural zoonotic cycle of transmission of infection between laboratory mice and laboratory-reared Ixodes ticks. To determine whether similar changes occur in the natural transmission cycle, we reisolated strain B31 from mouse skin at the fifth zoonotic cycle. This reisolated derivative had the same infectivity as the parent B31 strain, had lost the 8-kb supercoiled plasmid present in B31, and induced a gross serum antibody response indistinguishable from the B31 immune response. Analysis of antigen expression with monoclonal antibodies generated against B31, however, showed differential expression of a subset of antigens between B31 and the isolated derivative.
Starting Page	4795
File Format	PDF
ISSN	10985522
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	12
Volume Number	63
Language	English
Publisher Date	1995-12-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

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Variation in antigenicity and infectivity of derivatives of Borrelia burgdorferi, strain B31, maintained in the natural, zoonotic cycle compared with maintenance in culture.