NDLI: Rice dwarf mutant d1, which is defective in the α subunit of the heterotrimeric G protein, affects gibberellin signal transduction

Content Provider	PubMed Central
Author	Miyako, Ueguchi-tanaka Fujisawa, Yukiko Kobayashi, Masatomo Ashikari, Motoyuki Iwasaki, Yukimoto Kitano, Hidemi Matsuoka, Makoto
Copyright Year	2000
Abstract	Previously, we reported that the rice dwarf mutant, d1, is defective in the α subunit of the heterotrimeric G protein (Gα). In the present study, gibberellin (GA) signaling in d1 and the role of the Gα protein in the GA-signaling pathway were investigated. Compared with the wild type, GA induction of α-amylase activity in aleurone cells of d1 was greatly reduced. Relative to the wild type, the GA3-treated aleurone layer of d1 had lower expression of Ramy1A, which encodes α-amylase, and OsGAMYB, which encodes a GA-inducible transcriptional factor, and no increase in expression of Ca 2 + -ATPase. However, in the presence of high GA concentrations, α-amylase induction occurred even in d1. The GA sensitivity of second leaf sheath elongation in d1 was similar to that of the wild type in terms of dose responsiveness, but the response of internode elongation to GA was much lower in d1. Furthermore, Os20ox expression was up-regulated, and the GA content was elevated in the stunted internodes of d1. All these results suggest that d1 affects a part of the GA-signaling pathway, namely the induction of α-amylase in the aleurone layer and internode elongation. In addition, a double mutant between d1 and another GA-signaling mutant, slr, revealed that SLR is epistatic to the D1, supporting that the Gα protein is involved in GA signaling. However, the data also provide evidence for the presence of an alternative GA-signaling pathway that does not involve the Gα protein. It is proposed that GA signaling via the Gα protein may be more sensitive than that of the alternative pathway, as indicated by the low GA responsiveness of this Gα-independent pathway.
Related Links	http://dx.doi.org/10.1073/pnas.97.21.11638
Ending Page	11643
Page Count	6
Starting Page	11638
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	21
Volume Number	97
Language	English
Publisher	The National Academy of Sciences
Publisher Date	2000-10-10
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Rice dwarf mutant d1, which is defective in the α subunit of the heterotrimeric G protein, affects gibberellin signal transduction

The α-subunit of the rice heterotrimeric G protein, RGA1, regulates drought tolerance during the vegetative phase in the dwarf rice mutant d1

Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the α-subunit of GTP-binding protein

The heterotrimeric G protein α subunit acts upstream of the small GTPase Rac in disease resistance of rice

Expression profile of the α subunit of the heterotrimeric G protein in rice

Heterotrimeric G protein α subunit is involved in rice brassinosteroid response

Function of α subunit of heterotrimeric G protein in brassinosteroid response of rice plants

Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein α-subunit

Signal transduction by a nondissociable heterotrimeric yeast G protein

Rice dwarf mutant d1, which is defective in the α subunit of the heterotrimeric G protein, affects gibberellin signal transduction

Similar Documents

Rice dwarf mutant d1, which is defective in the α subunit of the heterotrimeric G protein, affects gibberellin signal transduction

The α-subunit of the rice heterotrimeric G protein, RGA1, regulates drought tolerance during the vegetative phase in the dwarf rice mutant d1

Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the α-subunit of GTP-binding protein

The heterotrimeric G protein α subunit acts upstream of the small GTPase Rac in disease resistance of rice

Expression profile of the α subunit of the heterotrimeric G protein in rice

Heterotrimeric G protein α subunit is involved in rice brassinosteroid response

Function of α subunit of heterotrimeric G protein in brassinosteroid response of rice plants

Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein α-subunit

Signal transduction by a nondissociable heterotrimeric yeast G protein

Rice dwarf mutant d1, which is defective in the α subunit of the heterotrimeric G protein, affects gibberellin signal transduction