NDLI: Effect of Sequence-Directed Nucleosome Disruption on Cell-Type-Specific Repression by α2/Mcm1 in the Yeast Genome▿

Content Provider	PubMed Central
Author	Morohashi, Nobuyuki Yamamoto, Yuichi Kuwana, Shunsuke Morita, Wataru Shindo, Heisaburo Mitchell, Aaron P. Shimizu, Mitsuhiro
Copyright Year	2006
Abstract	In Saccharomyces cerevisiae, a-cell-specific genes are repressed in MATα cells by α2/Mcm1, acting in concert with the Ssn6-Tup1 corepressors and the Isw2 chromatin remodeling complex, and nucleosome positioning has been proposed as one mechanism of repression. However, prior studies showed that nucleosome positioning is not essential for repression by α2/Mcm1 in artificial reporter plasmids, and the importance of the nucleosome positioning remains questionable. We have tested the function of positioned nucleosomes through alteration of genomic chromatin at the a-cell-specific gene BAR1. We report here that a positioned nucleosome in the BAR1 promoter is disrupted in cis by the insertion of diverse DNA sequences such as poly(dA) · poly(dT) and poly(dC-dG) · poly(dC-dG), leading to inappropriate partial derepression of BAR1. Also, we show that isw2 mutation causes loss of nucleosome positioning in BAR1 in MATα cells as well as partial disruption of repression. Thus, nucleosome positioning is required for full repression, but loss of nucleosome positioning is not sufficient to relieve repression completely. Even though disruption of nucleosome positioning by the cis- and trans-acting modulators of chromatin has a modest effect on the level of transcription, it causes significant degradation of the α-mating pheromone in MATα cells, thereby affecting its cell type identity. Our results illustrate a useful paradigm for analysis of chromatin structural effects at genomic loci.
Related Links	http://dx.doi.org/10.1128/ec.00105-06
Ending Page	1933
Page Count	9
Starting Page	1925
File Format	PDF
ISSN	15359778
e-ISSN	15359786
Journal	Eukaryotic Cell
Issue Number	11
Volume Number	5
Language	English
Publisher	American Society for Microbiology
Publisher Date	2006-11-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A nucleosome positioned by α2/Mcm1 prevents Hap1 activator binding in vivo

Both activation and repression of a-mating-type-specific genes in yeast require transcription factor Mcm1.

The role of nucleosome positioning in repression by the yeast α2/Mcm1p repressor

The yeast Mcm1 protein is regulated posttranscriptionally by the flux of glycolysis.

TFIIIB placement on a yeast U6 RNA gene in vivo is directed primarily by TFIIIC rather than by sequence-specific DNA contacts.

Specific repression of the yeast silent mating locus HMR by an adjacent telomere.

DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus laevis.

Sequence-specific transcriptional activation by Myc and repression by Max.

Biophysical Characterization of the Centromere-specific Nucleosome from Budding Yeast*

Effect of Sequence-Directed Nucleosome Disruption on Cell-Type-Specific Repression by α2/Mcm1 in the Yeast Genome▿

Similar Documents

A nucleosome positioned by α2/Mcm1 prevents Hap1 activator binding in vivo

Both activation and repression of a-mating-type-specific genes in yeast require transcription factor Mcm1.

The role of nucleosome positioning in repression by the yeast α2/Mcm1p repressor

The yeast Mcm1 protein is regulated posttranscriptionally by the flux of glycolysis.

TFIIIB placement on a yeast U6 RNA gene in vivo is directed primarily by TFIIIC rather than by sequence-specific DNA contacts.

Specific repression of the yeast silent mating locus HMR by an adjacent telomere.

DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus laevis.

Sequence-specific transcriptional activation by Myc and repression by Max.

Biophysical Characterization of the Centromere-specific Nucleosome from Budding Yeast*

Effect of Sequence-Directed Nucleosome Disruption on Cell-Type-Specific Repression by α2/Mcm1 in the Yeast Genome▿