NDLI: Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis

Content Provider	PubMed Central
Author	Maeno, Emi Ishizaki, Yasuki Kanaseki, Toku Hazama, Akihiro Okada, Yasunobu
Copyright Year	2000
Abstract	A major hallmark of apoptosis is normotonic shrinkage of cells. Here, we studied the relation between apoptotic cell shrinkage and apoptotic cell death. Induction of the apoptotic volume decrease (AVD) under normotonic conditions was found to be coupled to facilitation of the regulatory volume decrease (RVD), which is known to be attained by parallel operation of Cl− and K+ channels, under hypotonic conditions. Both the AVD induction and the RVD facilitation were found to precede cytochrome c release, caspase-3 activation, DNA laddering, and ultrastructural alterations in three cell types after apoptotic insults with two distinct apoptosis inducers. Also, the AVD was not prevented by a broad-spectrum caspase inhibitor. When the AVD induction and the RVD facilitation were prevented by blocking volume-regulatory Cl− or K+ channels, these cells did not show succeeding apoptotic biochemical and morphological events and were rescued from death. Thus, it is concluded that the AVD, which is caused by disordered cell volume regulation, is an early prerequisite to apoptotic events leading to cell death.
Starting Page	9487
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	17
Volume Number	97
Language	English
Publisher	The National Academy of Sciences
Publisher Date	2000-08-15
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis

The Apoptotic Volume Decrease Is an Upstream Event of MAP Kinase Activation during Staurosporine-Induced Apoptosis in HeLa Cells

Early-phase occurrence of K+ and Cl− efflux in addition to Ca2+ mobilization is a prerequisite to apoptosis in HeLa cells

Fas Splicing Regulation during Early Apoptosis Is Linked to Caspase-mediated Cleavage of U2AF65

Down-regulation of c-myb gene expression is a prerequisite for erythropoietin-induced erythroid differentiation.

Proliferation is a prerequisite for bacterial superantigen-induced T cell apoptosis in vivo

Hypertonicity-induced cation channels rescue cells from staurosporine-elicited apoptosis

The tumor-suppressor gene FHIT is involved in the regulation of apoptosis and in cell cycle control

An essential role for Bruton's [corrected] tyrosine kinase in the regulation of B-cell apoptosis.

Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis

Similar Documents

Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis

The Apoptotic Volume Decrease Is an Upstream Event of MAP Kinase Activation during Staurosporine-Induced Apoptosis in HeLa Cells

Early-phase occurrence of K+ and Cl− efflux in addition to Ca2+ mobilization is a prerequisite to apoptosis in HeLa cells

Fas Splicing Regulation during Early Apoptosis Is Linked to Caspase-mediated Cleavage of U2AF65

Down-regulation of c-myb gene expression is a prerequisite for erythropoietin-induced erythroid differentiation.

Proliferation is a prerequisite for bacterial superantigen-induced T cell apoptosis in vivo

Hypertonicity-induced cation channels rescue cells from staurosporine-elicited apoptosis

The tumor-suppressor gene FHIT is involved in the regulation of apoptosis and in cell cycle control

An essential role for Bruton's [corrected] tyrosine kinase in the regulation of B-cell apoptosis.

Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis