NDLI: Bacteriophage-triggered defense systems: phage adaptation and design improvements.

Please wait, while we are loading the content...

Year: 2016, Volume: 82

Year: 2015, Volume: 82

Year: 2015, Volume: 81

Year: 2014, Volume: 80

Year: 2013, Volume: 79

Year: 2012, Volume: 78

Year: 2011, Volume: 77

Year: 2010, Volume: 76

Year: 2009, Volume: 75

Year: 2008, Volume: 74

Year: 2007, Volume: 73

Year: 2006, Volume: 72

Year: 2005, Volume: 71

Year: 2004, Volume: 70

Year: 2003, Volume: 69

Year: 2002, Volume: 69

Year: 2002, Volume: 68

Year: 2001, Volume: 67

Year: 2000, Volume: 66

Year: 1999, Volume: 65

Year: 1998, Volume: 64

Year: 1997, Volume: 63

Year: 1997, Volume: 63, Issue: 12

Year: 1997, Volume: 63, Issue: 11

In Vitro Studies on Reductive Vinyl Chloride Dehalogenation by an Anaerobic Mixed Culture

Monitoring of an Alkaline 2,4,6-Trichlorophenol-Degrading Enrichment Culture by DNA Fingerprinting Methods and Isolation of the Responsible Organism, Haloalkaliphilic Nocardioides sp. Strain M6

A novel competence gene, comP, is essential for natural transformation of Acinetobacter sp. strain BD413.

Effects of lactobacilli on yeast-catalyzed ethanol fermentations.

Isolation of new bacterial species from drinking water biofilms and proof of their in situ dominance with highly specific 16S rRNA probes.

Effects of elevated dissolved CO2 levels on batch and continuous cultures of Aspergillus niger A60: an evaluation of experimental methods.

Inhibitory Effect of Solar Radiation on Thymidine and Leucine Incorporation by Freshwater and Marine Bacterioplankton

Purification and characterization of staphylococcin BacR1, a broad-spectrum bacteriocin.

Conversion of beta-methylbutyric acid to beta-hydroxy-beta-methylbutyric acid by Galactomyces reessii.

Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR.

Recovery of culturability of an HOCl-stressed population of Escherichia coli after incubation in phosphate buffer: resuscitation or regrowth?

Intracellular pH is a major factor in the induction of tolerance to acid and other stresses in Lactococcus lactis.

Biodegradation of the gasoline oxygenates methyl tert-butyl ether, ethyl tert-butyl ether, and tert-amyl methyl ether by propane-oxidizing bacteria.

Flow sorting of microorganisms for molecular analysis.

Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test.

Numerical dominance of a group of marine bacteria in the alpha-subclass of the class Proteobacteria in coastal seawater.

On-Line Monitoring of Nitrogenase Activity in Cyanobacteria by Sensitive Laser Photoacoustic Detection of Ethylene

Characterization of the lacticin 481 operon: the Lactococcus lactis genes lctF, lctE, and lctG encode a putative ABC transporter involved in bacteriocin immunity.

Evidence for signaling between the phytopathogenic fungus Pythium ultimum and Pseudomonas fluorescens F113: P. ultimum represses the expression of genes in P. fluorescens F113, resulting in altered ecological fitness.

Effects of dissolved organic carbon and salinity on bioavailability of mercury.

Degradation of the fluoroquinolone enrofloxacin by the brown rot fungus Gloeophyllum striatum: identification of metabolites.

Nucleoside Oxidase, a Hydrogen Peroxide-Forming Oxidase, from Flavobacterium meningosepticum

Indigo formation by microorganisms expressing styrene monooxygenase activity.

cis-trans isomerization of unsaturated fatty acids: cloning and sequencing of the cti gene from Pseudomonas putida P8.

Synechococcus diversity in the California current as seen by RNA polymerase (rpoC1) gene sequences of isolated strains.

Contribution of the Regulatory Gene lemA to Field Fitness of Pseudomonas syringae pv. syringae

Stabilization of apoglobin by low temperature increases yield of soluble recombinant hemoglobin in Escherichia coli.

Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum.

Identification of N2-fixing plant- and fungus-associated Azoarcus species by PCR-based genomic fingerprints.

Trehalose induces antagonism towards Pythium debaryanum in Pseudomonas fluorescens ATCC 17400.

Bacterial Oxidation of Methyl Bromide in Fumigated Agricultural Soils

Glycogen biosynthesis via UDP-glucose in the ruminal bacterium Prevotella bryantii B1(4).

Genetic diversity and expression of the [NiFe] hydrogenase large-subunit gene of Desulfovibrio spp. in environmental samples.

Bacteriophage-triggered defense systems: phage adaptation and design improvements.

A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells.

Reduction of Hexavalent Uranium from Organic Complexes by Sulfate- and Iron-Reducing Bacteria

Heterologous expression of the Mycobacterium tuberculosis gene encoding antigen 85A in Corynebacterium glutamicum.

Detection of low levels of enteric viruses in metropolitan and airplane sewage.

Improvement of Arbuscular Mycorrhiza Development by Inoculation of Soil with Phosphate-Solubilizing Rhizobacteria To Improve Rock Phosphate Bioavailability ((sup32)P) and Nutrient Cycling

Improvement of Bacillus sphaericus toxicity against dipteran larvae by integration, via homologous recombination, of the Cry11A toxin gene from Bacillus thuringiensis subsp. israelensis.

Differentiation of Erwinia amylovora strains by pulsed-field gel electrophoresis.

Geographical Differentiation of the Population of Xanthomonas axonopodis pv. manihotis in Colombia

Evidence That Ceriporiopsis subvermispora Degrades Nonphenolic Lignin Structures by a One-Electron-Oxidation Mechanism

Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR.

Contribution of the 65-kilodalton protein encoded by the cloned gene cry19A to the mosquitocidal activity of Bacillus thuringiensis subsp. jegathesan.

Recombinant luminescent bacteria for measuring bioavailable arsenite and antimonite.

Development of a diagnostic DNA probe for xanthomonads causing bacterial spot of peppers and tomatoes.

Influence of Oxygen and Glucose on Primary Metabolism and Astaxanthin Production by Phaffia rhodozyma in Batch and Fed-Batch Cultures: Kinetic and Stoichiometric Analysis

Phenotypic and Genotypic Comparison of Symbiotic and Free-Living Cyanobacteria from a Single Field Site

Biodiversity of a Burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages.

Association of mercury resistance with antibiotic resistance in the gram-negative fecal bacteria of primates.

Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to PCR.

Biodegradation of [(sup14)C]Benzo[a]pyrene Added in Crude Oil to Uncontaminated Soil

Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA.

Metal resistance in Acidocella strains and plasmid-mediated transfer of this characteristic to Acidiphilium multivorum and Escherichia coli.

Imaging of Lactobacillus brevis single cells and microcolonies without a microscope by an ultrasensitive chemiluminescent enzyme immunoassay with a photon-counting television camera.

Development and application of monoclonal antibodies for in situ detection of indigenous bacterial strains in aquatic ecosystems.

Green fluorescent protein as a marker for Pseudomonas spp.

Inhibition of Methanogenesis by Methyl Fluoride: Studies of Pure and Defined Mixed Cultures of Anaerobic Bacteria and Archaea

Specific detection and confirmation of Campylobacter jejuni by DNA hybridization and PCR.

Comparison of PCR and Plaque Assay for Detection and Enumeration of Coliphage in Polluted Marine Waters

Degradation of 4-Chlorophenol via the meta Cleavage Pathway by Comamonas testosteroni JH5

Occurrence of Flavonoids and Nucleosides in Agricultural Soils

Detection of Activity Responsible for Induction of the Agrobacterium tumefaciens Virulence Genes in Bacteriological Agar

Controlled gene expression systems for lactic acid bacteria: transferable nisin-inducible expression cassettes for Lactococcus, Leuconostoc, and Lactobacillus spp.

Virulence factors of verocytotoxin-producing Escherichia coli isolated from raw meats.

Regulation of the Synthesis and Activity of Ammonia Monooxygenase in Nitrosomonas europaea by Altering pH To Affect NH(inf3) Availability

Application of the extracellular alpha-amylase gene from Streptococcus bovis 148 to construction of a secretion vector for yogurt starter strains.

Combined Light Microscopy and Attenuated Total Reflection Fourier Transform Infrared Spectroscopy for Integration of Biofilm Structure, Distribution, and Chemistry at Solid-Liquid Interfaces

A relative of the broad-host-range plasmid RSF1010 detected in Erwinia amylovora.

Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry.

Small-subunit rRNA genes and in situ hybridization with oligonucleotides specific for the bacterial symbionts in the larvae of the bryozoan Bugula neritina and proposal of "Candidatus endobugula sertula".

Methane and trichloroethylene oxidation by an estuarine methanotroph, Methylobacter sp. strain BB5.1.

Evidence that elevated CO2 levels can indirectly increase rhizosphere denitrifier activity.

Evidence for Intracellular and Extracellular Dimethylsulfoniopropionate (DMSP) Lyases and DMSP Uptake Sites in Two Species of Marine Bacteria

Inactivation of Cryptosporidium parvum Oocysts and Clostridium perfringens Spores by a Mixed-Oxidant Disinfectant and by Free Chlorine

A Rapid and Simple Method for Estimating Sulfate Reduction Activity and Quantifying Inorganic Sulfides

Year: 1997, Volume: 63, Issue: 10

Year: 1997, Volume: 63, Issue: 9

Year: 1997, Volume: 63, Issue: 8

Year: 1997, Volume: 63, Issue: 7

Year: 1997, Volume: 63, Issue: 6

Year: 1997, Volume: 63, Issue: 5

Year: 1997, Volume: 63, Issue: 4

Year: 1997, Volume: 63, Issue: 3

Year: 1997, Volume: 63, Issue: 2

Year: 1997, Volume: 63, Issue: 1

Year: 1996, Volume: 62

Year: 1995, Volume: 61

Year: 1994, Volume: 60

Year: 1993, Volume: 59

Year: 1992, Volume: 58

Year: 1991, Volume: 57

Year: 1990, Volume: 56

Year: 1989, Volume: 55

Year: 1988, Volume: 54

Year: 1987, Volume: 53

Year: 1986, Volume: 52

Year: 1986, Volume: 51

Year: 1985, Volume: 50

Year: 1985, Volume: 49

Year: 1984, Volume: 48

Year: 1984, Volume: 47

Year: 1983, Volume: 46

Year: 1983, Volume: 45

Year: 1982, Volume: 44

Year: 1982, Volume: 43

Year: 1981, Volume: 42

Year: 1981, Volume: 41

Year: 1980, Volume: 40

Year: 1980, Volume: 39

Year: 1979, Volume: 38

Year: 1979, Volume: 37

Year: 1978, Volume: 36

Year: 1978, Volume: 35

Year: 1977, Volume: 34

Year: 1977, Volume: 33

Year: 1976, Volume: 32

Year: 1976, Volume: 31

Bacteriophage-triggered defense systems: phage adaptation and design improvements.

Content Provider	PubMed Central
Author	Djordjevic, G. M. Klaenhammer, T. R.
Abstract	A novel bacteriophage defense system, based on an inducible suicide gene, was challenged with a lactococcal bacteriophage to investigate the potential for phage adaptation. The defense system was encoded by pTRK414H, a high-copy-number replicon encoding a tightly regulated phi 31p trigger promoter fused to the lethal LlaIR+ restriction endonuclease cassette. Repeated transfers of Lactococcus lactis NCK690(pTRK414H) in the presence of phi 31 selected for phage phi 31 derivatives which were markedly less sensitive to phi 31p-LlaIR(+)-encoded restriction than the parental phage, phi 31. The efficiency of plaquing (EOP) on L. lactis NCK690(pTRK414H) was 10(-4) for phi 31 versus 0.4 for the derived phages. The mutant phages remained fully sensitive to LlaIR+ restriction, suggesting an alteration in the recognition or firing of the phi 31p promoter. Sequencing over the promoter region in four mutant phages revealed the identical C-to-A transversion, generating a Phe-to-Leu substitution, in a transcriptional activator of the phi 31p promoter, designated ORF2. The mutant phages were analyzed for their ability to induce the native phi 31p promoter element fused to a lacZst reporter gene. Compared to the parental phage, phi 31, lower levels of beta-galactosidase activity were induced throughout the lytic cycle, indicating that the strength at which the mutant phages activated the phi 31p promoter was altered. Based on these observations, improvements were made in promoter strength and restriction activity in an attempt to elevate the effectiveness of the phage-triggered suicide system. When the phi 31p-LlaIR+ cassette was paired with other abortive defense systems, Per31 and AbiA, the EOP of phi 31 was reduced to < 10(-10) and the level of phage in the culture was lowered below the detection limits of the assay.
Starting Page	4370
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	11
Volume Number	63
Language	English
Publisher Date	1997-11-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in