NDLI: Combinatorial Interaction of Cis Elements Specifies the Expression of the Arabidopsis AtHsp90-1 Gene1

Content Provider	PubMed Central
Author	Kosmas, Haralampidis Milioni, Dimitra Rigas, Stamatis Hatzopoulos, Polydefkis
Copyright Year	2002
Abstract	The promoter region of the Arabidopsis AtHsp90-1 gene is congested with heat shock elements and stress response elements, as well as with other potential transcriptional binding sites (activating protein 1, CCAAT/enhancer-binding protein element, and metal regulatory element). To determine how the expression of this bona fide AtHsp90-1 gene is regulated, a comprehensive quantitative and qualitative promoter deletion analysis was conducted under various environmental conditions and during development. The promoter induces gene expression at high levels after heat shock and arsenite treatment. However, our results show that the two stress responses may involve common but not necessarily the same regulatory elements. Whereas for heat induction, heat shock elements and stress response elements act cooperatively to promote high levels of gene expression, arsenite induction seems to require the involvement of activating protein 1 regulatory sequences. In stressed transgenic plants harboring the full-length promoter, β-glucuronidase activity was prominent in all tissues. Nevertheless, progressive deletion of the promoter decreases the level of expression under heat shock and restricts it predominantly in the two meristems of the plant. In contrast, under arsenite induction, proximal sequences induce AtHsp90-1 gene expression only in the shoot meristem. Distally located elements negatively regulate AtHsp90-1 gene expression under unstressed conditions, whereas flower-specific regulated expression in mature pollen grains suggests the prominent role of the AtHsp90-1 in pollen development. The results show that the regulation of developmental expression, suppression, or stress induction is mainly due to combinatorial contribution of the cis elements in the promoter region of the AtHsp90-1 gene.
Related Links	http://dx.doi.org/10.1104/pp.004044
Ending Page	1149
Page Count	12
Starting Page	1138
File Format	PDF
ISSN	00320889
e-ISSN	15322548
Journal	Plant Physiology
Issue Number	3
Volume Number	129
Language	English
Publisher	American Society of Plant Physiologists
Publisher Date	2002-07-01
Access Restriction	Open
Rights Holder	American Society of Plant Physiologists
Subject Keyword	Plant Science Genetics Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Physiology Plant Science

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