NDLI: Spatiotemporal patterning of IP3-mediated Ca2+ signals in Xenopus oocytes by Ca2+-binding proteins

Content Provider	PubMed Central
Author	Dargan, Sheila L. Schwaller, Beat Parker, Ian
Copyright Year	2004
Abstract	Ca2+-binding proteins (CaBPs) are expressed in a highly specific manner across many different cell types, yet the physiological basis underlying their selective distribution patterns remains unclear. We used confocal line-scan microscopy together with photo-release of IP3 in Xenopus oocytes to investigate the actions of mobile cytosolic CaBPs on the spatiotemporal properties of IP3-evoked Ca2+ signals. Parvalbumin (PV), a CaBP with slow Ca2+-binding kinetics, shortened the duration of IP3-evoked Ca2+ signals and ‘balkanized’ global responses into discrete localized events (puffs). In contrast, calretinin (CR), a presumed fast buffer, prolonged Ca2+ responses and promoted ‘globalization’ of spatially uniform Ca2+ signals at high [IP3]. Oocytes loaded with CR or PV showed Ca2+ puffs following photolysis flashes that were subthreshold in controls, and the spatiotemporal properties of these localized events were differentially modulated by PV and CR. In comparison to results we previously obtained with exogenous Ca2+ buffers, PV closely mimicked the actions of the slow buffer EGTA, whereas CR showed important differences from the fast buffer BAPTA. Most notably, puffs were never observed after loading BAPTA, and this exogenous buffer did not show the marked sensitization of IP3 action evident with CR. The ability of Ca2+ buffers and CaBPs with differing kinetics to fine-tune both global and local intracellular Ca2+ signals is likely to have significant physiological implications.
Related Links	http://dx.doi.org/10.1113/jphysiol.2003.059204
Starting Page	447
File Format	PDF
ISSN	14697793
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 2
Volume Number	556
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2004-04-15
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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Spatiotemporal patterning of IP3-mediated Ca2+ signals in Xenopus oocytes by Ca2+-binding proteins

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Spatiotemporal patterning of IP3-mediated Ca2+ signals in Xenopus oocytes by Ca2+-binding proteins

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