NDLI: A major role for the TATA box in recruitment of chromatin modifying complexes to a globin gene promoter

Content Provider	PubMed Central
Author	Gui, Chang-yun Dean, Ann
Copyright Year	2003
Abstract	The developmentally regulated mammalian β-globin genes are activated by a distant locus control region/enhancer. To understand the role of chromatin remodeling complexes in this activation, we used stably replicated chromatin templates, in which transcription activation of the human embryonic ε-globin gene depends on the tandem Maf-recognition elements (MAREs) within the β-globin locus control region HS2 enhancer, to which the erythroid factor NF-E2 binds. The HS2 MAREs are required for nucleosome mobilization and histone hyperacetylation at the distant promoter. Nucleosome mobilization also requires the promoter TATA box, and is independent of histone hyperacetylation. In contrast, promoter hyperacetylation requires the promoter GATA-1, and CACC-factor activator motifs, as well as the TATA box. ChIP analysis reveals that NF-E2 is associated with the active ε-globin promoter, which lacks an NF-E2 binding sequence, in a TATA box and HS2/MARE-dependent fashion. NF-E2 association with the ε-globin promoter coincides with that of RNA polymerase II at both regulatory sites. The results emphasize MARE–TATA box interactions in the recruitment of complexes modifying promoter chromatin for transcription activation and imply close physical interaction between widely separated regulatory sequences mediated through these sites.
Related Links	http://dx.doi.org/10.1073/pnas.1236499100
Ending Page	7014
Page Count	6
Starting Page	7009
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	12
Volume Number	100
Language	English
Publisher	National Academy of Sciences
Publisher Date	2003-06-10
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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