NDLI: Photoreactivation of DNA by an Archaeal nucleoprotein Sso7d

Content Provider	PubMed Central
Author	Tashiro, Ryu Wang, Andrew H. J. Sugiyama, Hiroshi
Copyright Year	2006
Abstract	Sso7d is a chromosomal protein of hyperthermophilic Archaea. The crystal structure of Sso7d–d(GTAATIUAC)2 has been clarified at high resolution, showing that the protein binds in the minor groove of DNA, causing a sharp kink of ≈60°. Recently, we found that photoirradiation of Sso7d and 5-iodouracil-(IU)-containing 5′-d(GTAATIUAC)-3′ efficiently induced the abstraction of hydrogen from the methyl group of T5 at the kink. In the present study, we found that the photoreactivity of 5-bromouracil (BrU)-containing 5′-d(GTAATBrUAC)-3′ was enhanced in the presence of Sso7d. Using hypoxanthine (I)-containing 5′-d(ITAATBrUAC)-3′, we demonstrated that electron transfer occurs efficiently from Sso7d to DNA. Product analysis showed that Trp-24 of Sso7d, located at the surface of the DNA, is consumed to produce N′-formylkynurenine during photoirradiation, indicating that Trp-24 acts as an electron source. To explore the possibility of electron transfer between Sso7d and other DNA substrates, we examined the photochemical repair of the thymine dimer 5′-d(GTAAT<>TAC)-3′ by Sso7d. Sso7d effectively repaired 5′-d(GTAAT<>TAC)-3′ to 5′-d(GTAATTAC)-3′ under irradiation conditions. During this reaction, Trp-24 was not oxidized significantly, indicating that the anion radical of the repaired TT sequence is oxidized by the cation radical of Trp-24, and that a so-called “circular electron transfer” mechanism is operating in this system.
Related Links	http://dx.doi.org/10.1073/pnas.0603484103
Ending Page	16659
Page Count	5
Starting Page	16655
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	45
Volume Number	103
Language	English
Publisher	National Academy of Sciences
Publisher Date	2006-11-07
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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