NDLI: Tissue kallikrein stimulates Ca2+ reabsorption via PKC-dependent plasma membrane accumulation of TRPV5

Content Provider	PubMed Central
Author	Gkika, Dimitra Topala, Catalin N. Chang, Qing Picard, Nicolas Thébault, Stéphanie Pascal, Houillier Hoenderop, Joost G. J. Bindels, René J. M.
Copyright Year	2006
Abstract	The transient receptor potential vanilloid 5 (TRPV5) channel determines urinary Ca2+ excretion, and is therefore critical for Ca2+ homeostasis. Interestingly, mice lacking the serine protease tissue kallikrein (TK) exhibit robust hypercalciuria comparable to the Ca2+ leak in TRPV5 knockout mice. Here, we delineated the molecular mechanism through which TK stimulates Ca2+ reabsorption. Using TRPV5-expressing primary cultures of renal Ca2+-transporting epithelial cells, we showed that TK activates Ca2+ reabsorption. The stimulatory effect of TK was mimicked by bradykinin (BK) and could be reversed by application of JE049, a BK receptor type 2 antagonist. A cell permeable analog of DAG increased TRPV5 activity within 30 min via protein kinase C activation of the channel since mutation of TRPV5 at the putative PKC phosphorylation sites S299 and S654 prevented the stimulatory effect of TK. Cell surface labeling revealed that TK enhances the amount of wild-type TRPV5 channels, but not of the TRPV5 S299A and S654A mutants, at the plasma membrane by delaying its retrieval. In conclusion, TK stimulates Ca2+ reabsorption via the BK-activated PLC/DAG/PKC pathway and the subsequent stabilization of the TRPV5 channel at the plasma membrane.
Related Links	http://dx.doi.org/10.1038/sj.emboj.7601357
Ending Page	4716
Page Count	10
Starting Page	4707
File Format	PDF
ISSN	02614189
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	20
Volume Number	25
Language	English
Publisher Date	2006-10-18
Access Restriction	Open
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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