NDLI: Nuclear import in permeabilized protoplasts from higher plants has unique features.

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Nuclear import in permeabilized protoplasts from higher plants has unique features.

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Nuclear import in permeabilized protoplasts from higher plants has unique features.

Content Provider	PubMed Central
Author	Hicks, G. R. Smith, H. M. Lobreaux, S. Raikhel, N. V.
Abstract	The import of proteins into the nucleus is a poorly understood process that is thought to require soluble cytosolic factors in vertebrates and yeast. To test this model in plants and to identify components of the import apparatus, we developed a direct in vitro nuclear import assay by using tobacco protoplasts that were permeabilized without detergents such as digitonin or Triton X-100. Substrates were imported specifically by a mechanism that required only guanine nucleotides. Moreover, in vitro import did not require exogenous cytosol. To investigate this novel finding, we isolated a full-length cDNA encoding an Arabidopsis homolog of vertebrate and yeast nuclear localization signal receptors and produced an affinity-purified antibody. The plant receptor was tightly associated with cellular components in permeabilized protoplasts, even in the presence of 0.1% Triton X-100, indicating that this factor and probably others were retained to an extent sufficient to support import. The lectin wheat germ agglutinin bound to the nucleus; however, it did not block translocation in our system, indicating that direct interaction with polysaccharide modifications at the nuclear pore complex was probably not essential for import in plants. Other features of in vitro import included reduced but significant import at low temperature.
Starting Page	1337
File Format	PDF
ISSN	1532298X
e-ISSN	1532298X
Journal	The Plant Cell
Issue Number	8
Volume Number	8
Language	English
Publisher Date	1996-08-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science

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