NDLI: Size and Structure of Replicating Mitochondrial DNA in Cultured Tobacco Cells.

Content Provider	PubMed Central
Author	Oldenburg, Dj Bendich, Aj
Abstract	The BY-2 tobacco cell line was used to study the size and structure of replicating mitochondrial DNA (mtDNA). Approximately 70 to 90% of the newly synthesized mtDNA did not migrate during pulsed-field gel electrophoresis. Moving pictures of the fluorescently labeled molecules showed that most of the immobile well-bound DNA was in structures larger than the size of the BY-2 mitochondrial genome of ~270 kb. Most of the structures appeared as complex forms with multiple DNA fibers. The sizes of the circular molecules that were also observed ranged continuously from ~20 to 560 kb without prominent size classes. Pulse-chase and mung bean nuclease experiments showed that the well-bound DNA contained single-stranded regions and was converted to linear molecules of between 50 and 150 kb. MtDNA replication in plants may be initiated by recombination events that create branched structures of multigenomic concatemers that are then processed to 50- to 150-kb subgenomic fragments.
Starting Page	447
File Format	PDF
ISSN	1532298X
e-ISSN	1532298X
Journal	The Plant Cell
Issue Number	3
Volume Number	8
Language	English
Publisher Date	1996-03-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science

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Size and Structure of Replicating Mitochondrial DNA in Cultured Tobacco Cells.