NDLI: Identification of a Novel Galactosyl Transferase Involved in Biosynthesis of the Mycobacterial Cell Wall

Content Provider	PubMed Central
Author	Mikušová, Katarína Martina, Beláňová Jana, Korduláková Honda, Kristine Mcneil, Michael R. Mahapatra, Sebabrata Crick, Dean C. Brennan, Patrick J.
Copyright Year	2006
Abstract	The possibility of the Rv3782 protein of Mycobacterium tuberculosis being a putative galactosyl transferase (GalTr) implicated in galactan synthesis arose from its similarity to the known GalTr Rv3808c, its classification as a nucleotide sugar-requiring inverting glycosyltransferase (GT-2 family), and its location within the “possible arabinogalactan biosynthetic gene cluster” of M. tuberculosis. In order to study the function of the enzyme, active membrane and cell wall fractions from Mycobacterium smegmatis containing the overexpressed Rv3782 protein were incubated with endogenous decaprenyldiphosphoryl-N-acetylglucosaminyl-rhamnose (C50-P-P-GlcNAc-Rha) as the primary substrate for galactan synthesis and UDP-[14C]galactopyranose as the immediate precursor of UDP-[14C]galactofuranose, the ultimate source of all of the galactofuranose (Galf) units of galactan. Obvious increased and selective synthesis of C50-P-P-GlcNAc-Rha-Galf-Galf, the earliest product in the pathway leading to the fully polymerized galactan, was observed, suggesting that Rv3782 encodes a GalTr involved in the first stages of galactan synthesis. Time course experiments pointed to a possible bifunctional enzyme responsible for the initial synthesis of C50-P-P-GlcNAc-Rha-Galf, followed by immediate conversion to C50-P-P-GlcNAc-Rha-Galf-Galf. Thus, Rv3782 appears to be the initiator of galactan synthesis, while Rv3808c continues with the subsequent polymerization events.
Related Links	http://dx.doi.org/10.1128/JB.00489-06
Starting Page	6592
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	18
Volume Number	188
Language	English
Publisher	American Society for Microbiology
Publisher Date	2006-09-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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