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| Content Provider | PubMed Central |
|---|---|
| Author | Shaw, Lindsey N. Aish, Joanne Davenport, Jessica E. Brown, Melanie C. Lithgow, James K. Simmonite, Kay Crossley, Howard Travis, James Potempa, Jan Foster, Simon J. |
| Copyright Year | 2006 |
| Abstract | The commonly used Staphylococcus aureus laboratory strain 8325-4 bears a naturally occurring 11-bp deletion in the σB-regulating phosphatase rsbU. We have previously published a report (M. J. Horsburgh, J. L. Aish, I. J. White, L. Shaw, J. K. Lithgow, and S. J. Foster, J. Bacteriol. 184:5457-5467, 2002) on restoring the rsbU deletion, producing a σB-functional 8325-4 derivative, SH1000. SH1000 is pleiotropically altered in phenotype from 8325-4, displaying enhanced pigmentation, increased growth yields, and a marked decrease in secreted exoproteins. This reduction in exoprotein secretion appears to result from a sixfold reduction in agr expression. In this study we have undertaken transposon mutagenesis of SH1000 to identify components involved in the modulation of extracellular proteases and α-hemolysin compared to 8325-4. In total, 13 genes were identified displaying increased α-hemolysin transcription and extracellular proteolysis. Phenotypic analysis revealed that each mutant also had decreased pigmentation and a general increase in protein secretion. Interestingly this phenotype was not identical in each case but was variable from mutant to mutant. None of the genes identified encoded classic regulatory proteins but were predominantly metabolic enzymes involved in amino acid biosynthesis and transport. Further analysis revealed that all of these mutations were clustered in a 35-kb region of the chromosome. By complementation and genetic manipulation we were able to demonstrate the validity of these mutations. Interestingly transcriptional analysis revealed that rather than being regulated by σB, these genes appeared to have a role in the regulation of σB activity. Thus, we propose that the loss of individual genes in this chromosomal hot spot region results in a destabilization of cellular harmony and disruption of the σB regulatory cascade. |
| Related Links | http://dx.doi.org/10.1128/jb.00551-06 |
| Ending Page | 6080 |
| Page Count | 11 |
| Starting Page | 6070 |
| File Format | |
| ISSN | 00219193 |
| e-ISSN | 10985530 |
| Journal | Journal of Bacteriology |
| Issue Number | 17 |
| Volume Number | 188 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 2006-09-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Molecular Biology Microbiology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Microbiology |
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