NDLI: Locus ‘decontraction’ and centromeric recruitment contribute to allelic exclusion of the immunoglobulin heavy-chain gene

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Locus ‘decontraction’ and centromeric recruitment contribute to allelic exclusion of the immunoglobulin heavy-chain gene

Content Provider	PubMed Central
Author	Roldán, Esther Fuxa, Martin Chong, Winnie Martinez, Dolores Maria, Novatchkova Meinrad, Busslinger Skok, Jane A.
Abstract	Allelic exclusion of immunoglobulin genes ensures the expression of a single antibody molecule in B cells through mostly unknown mechanisms. Large-scale contraction of the immunoglobulin heavy-chain (Igh) locus facilitates rearrangements between Igh variable (VH) and diversity gene segments in pro–B cells. Here we show that these long-range interactions are mediated by ‘looping’ of individual Igh subdomains. The Igk locus also underwent contraction by looping in small pre–B and immature B cells, demonstrating that immunoglobulin loci are in a contracted state in rearranging cells. Successful Igh recombination induced the rapid reversal of locus contraction in response to pre–B cell receptor signaling, which physically separated the distal VH genes from the proximal Igh domain, thus preventing further rearrangements. In the absence of locus contraction, only the four most proximal VH genes escaped allelic exclusion in immature μ-transgenic B lymphocytes. Pre–B cell receptor signaling also led to rapid repositioning of one Igh allele to repressive centromeric domains in response to downregulation of interleukin 7 signaling. These data link both locus ‘decontraction’ and centromeric recruitment to the establishment of allelic exclusion at the Igh locus.
Related Links	http://dx.doi.org/10.1038/ni1150
Ending Page	41
Page Count	11
Starting Page	31
File Format	PDF
ISSN	15292908
e-ISSN	15292916
Journal	Nature immunology
Issue Number	1
Volume Number	6
Language	English
Publisher Date	2005-01-01
Access Restriction	Open
Subject Keyword	Immunology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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