NDLI: The mechanism of cryoinjury: In vitro studies on human internal mammary arteries

Content Provider	PubMed Central
Author	Else, Müller-schweinitzer Brett, Wolfgang Zerkowski, Hans-reinhard Haefeli, Walter E.
Copyright Year	2000
Abstract	The mechanism of cryoinjury was investigated in human internal mammary arteries (IMA) by monitoring contractile responses to ET-1 and KCl. For cryopreservation segments of IMA were equilibrated for 20 min with the cryomedium (RPMI 1640 culture medium containing 1.8 M DMSO and 0.1 M sucrose), frozen at a mean cooling rate of 1.3°C min−1 to −70°C and stored in liquid nitrogen. Before use, samples were thawed slowly and the cryomedium removed by dilution. Compared to unfrozen controls, ET-1 stimulated frozen/thawed IMA with similar efficacy but at 3 fold lower concentrations (P<0.001). Addition of ET-1 (100 nM) induced maximal contraction of unfrozen IMA within 10 min, declining thereafter to 25% after 90 min. In frozen/thawed IMA the ET-1-induced contraction was sustained but could be reversed if protein kinase C was blocked by staurosporine (100 nM). Responses to ET-1 of cryostored IMA were 5 fold more susceptible to blockade by nifedipine than those of controls. After cryostorage the efficacy of KCl was diminished to 40% (P<0.05) and the KCl curve was shifted to the left (2 fold, P<0.001). In both unfrozen and cryostored IMA the KCl (60 mM) effect was sustained and equally susceptible to nifedipine. It is suggested that the smooth muscle cell of IMA is receptive to physical forces which occur during cryopreservation. These forces modify transmembrane signal transduction and intracellular pathways, that are common to pharmacological agonists thereby changing vascular responses to several contractile agonists after thawing.
Related Links	http://dx.doi.org/10.1038/sj.bjp.0703326
Ending Page	640
Page Count	5
Starting Page	636
File Format	PDF
ISSN	00071188
Journal	British Journal of Pharmacology
Issue Number	3
Volume Number	130
Language	English
Publisher Date	2000-06-01
Access Restriction	Open
Subject Keyword	Pharmacology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Pharmacology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The mechanism of cryoinjury: In vitro studies on human internal mammary arteries.

Dual pathway for angiotensin II formation in human internal mammary arteries

Vasoconstrictor activity of novel endothelin peptide, ET-1(1 – 31), in human mammary and coronary arteries in vitro

Modulatory role of the vascular endothelium in the contractility of human isolated internal mammary artery.

The comparison of the responsiveness of human isolated internal mammary and gastroepiploic arteries to levcromakalim: an alternative approachto the management of graft spasm

Autoradiographic localization and function of beta-adrenoceptors on the human internal mammary artery and saphenous vein.

Comparison of vasodilators in human internal mammary artery: ghrelin is a potent physiological antagonist of endothelin-1.

Comparative genome-wide transcriptional analysis of human left and right internal mammary arteries

The 5-series F(2)-isoprostanes possess no vasomotor effects in the rat thoracic aorta, the human internal mammary artery and the human saphenous vein.

The mechanism of cryoinjury: In vitro studies on human internal mammary arteries

Similar Documents

The mechanism of cryoinjury: In vitro studies on human internal mammary arteries.

Dual pathway for angiotensin II formation in human internal mammary arteries

Vasoconstrictor activity of novel endothelin peptide, ET-1(1 – 31), in human mammary and coronary arteries in vitro

Modulatory role of the vascular endothelium in the contractility of human isolated internal mammary artery.

The comparison of the responsiveness of human isolated internal mammary and gastroepiploic arteries to levcromakalim: an alternative approachto the management of graft spasm

Autoradiographic localization and function of beta-adrenoceptors on the human internal mammary artery and saphenous vein.

Comparison of vasodilators in human internal mammary artery: ghrelin is a potent physiological antagonist of endothelin-1.

Comparative genome-wide transcriptional analysis of human left and right internal mammary arteries

The 5-series F(2)-isoprostanes possess no vasomotor effects in the rat thoracic aorta, the human internal mammary artery and the human saphenous vein.

The mechanism of cryoinjury: In vitro studies on human internal mammary arteries