NDLI: Secretion of L-glutamate from osteoclasts through transcytosis

Content Provider	PubMed Central
Author	Morimoto, Riyo Uehara, Shunsuke Yatsushiro, Shouki Juge, Narinobu Hua, Zhaolin Senoh, Shigenori Echigo, Noriko Hayashi, Mitsuko Mizoguchi, Toshihide Ninomiya, Tadashi Udagawa, Nobuyuki Hiroshi, Omote Yamamoto, Akitsugu Edwards, Robert H. Moriyama, Yoshinori
Copyright Year	2006
Abstract	Osteoclasts are involved in the catabolism of the bone matrix and eliminate the resulting degradation products through transcytosis, but the molecular mechanism and regulation of transcytosis remain poorly understood. Upon differentiation, osteoclasts express vesicular glutamate transporter 1 (VGLUT1), which is essential for vesicular storage and subsequent exocytosis of glutamate in neurons. VGLUT1 is localized in transcytotic vesicles and accumulates L-glutamate. Osteoclasts secrete L-glutamate and the bone degradation products upon stimulation with KCl or ATP in a Ca2+-dependent manner. KCl- and ATP-dependent secretion of L-glutamate was absent in osteoclasts prepared from VGLUT1−/− knockout mice. Osteoclasts express mGluR8, a class III metabotropic glutamate receptor. Its stimulation by a specific agonist inhibits secretion of L-glutamate and bone degradation products, whereas its suppression by a specific antagonist stimulates bone resorption. Finally, it was found that VGLUT1−/− mice develop osteoporosis. Thus, in bone-resorbing osteoclasts, L-glutamate and bone degradation products are secreted through transcytosis and the released L-glutamate is involved in autoregulation of transcytosis. Glutamate signaling may play an important role in the bone homeostasis.
Related Links	http://dx.doi.org/10.1038/sj.emboj.7601317
Ending Page	4186
Page Count	12
Starting Page	4175
File Format	PDF
ISSN	02614189
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	18
Volume Number	25
Language	English
Publisher Date	2006-09-20
Access Restriction	Open
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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