NDLI: Anti-mitochondrial antibody IgG subclass distribution and affinity in primary biliary cirrhosis.

Content Provider	PubMed Central
Author	Zhang, L. Weetman, A. P. Jayne, D. R. Yeaman, S. J. Bassendine, M. F. Oliveira, D. B. Turner, I.
Abstract	We have studied the total IgG subclass and anti-mitochondrial antibody (AMA) specific IgG subclass distribution in primary biliary cirrhosis (PBC) sera. In order to solve the problems caused by the differing affinities of subclass specific monoclonals and the competitive inhibition of antibodies in a whole serum assay, six sera were separated into subclass-specific fractions by affinity-depletion chromatography. AMA subclass distribution of 20 further sera from patients with PBC was assessed using conventional methods and the results were calibrated against one of the fractionated sera. Light chain distribution and AMA functional affinity were also assessed for the fractionated subclasses. Total amounts of IgG3 were significantly increased compared with normal controls. AMA were found in all IgG subclasses and not restricted predominantly to IgG3 as previously described. The functional affinity of IgG3 AMA is generally lower as compared with that of other subclasses. No light chain restriction was found.
Starting Page	56
File Format	PDF
ISSN	13652249
e-ISSN	13652249
Journal	Clinical and Experimental Immunology
Issue Number	1
Volume Number	88
Language	English
Publisher Date	1992-04-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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Anti-mitochondrial antibody IgG subclass distribution and affinity in primary biliary cirrhosis.