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| Content Provider | PubMed Central |
|---|---|
| Author | Urakawa, Hidetoshi Said, El Fantroussi Smidt, Hauke Smoot, James C. Tribou, Erik H. Kelly, John J. Noble, Peter A. Stahl, David A. |
| Copyright Year | 2003 |
| Abstract | The discrimination between perfect-match and single-base-pair-mismatched nucleic acid duplexes was investigated by using oligonucleotide DNA microarrays and nonequilibrium dissociation rates (melting profiles). DNA and RNA versions of two synthetic targets corresponding to the 16S rRNA sequences of Staphylococcus epidermidis (38 nucleotides) and Nitrosomonas eutropha (39 nucleotides) were hybridized to perfect-match probes (18-mer and 19-mer) and to a set of probes having all possible single-base-pair mismatches. The melting profiles of all probe-target duplexes were determined in parallel by using an imposed temperature step gradient. We derived an optimum wash temperature for each probe and target by using a simple formula to calculate a discrimination index for each temperature of the step gradient. This optimum corresponded to the output of an independent analysis using a customized neural network program. These results together provide an experimental and analytical framework for optimizing mismatch discrimination among all probes on a DNA microarray. |
| Related Links | http://dx.doi.org/10.1128/aem.69.5.2848-2856.2003 |
| Ending Page | 2856 |
| Page Count | 9 |
| Starting Page | 2848 |
| File Format | |
| ISSN | 00992240 |
| e-ISSN | 10985336 |
| Journal | Applied and Environmental Microbiology |
| Issue Number | 5 |
| Volume Number | 69 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 2003-05-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Biotechnology Food Science Ecology Applied Microbiology and Biotechnology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ecology Food Science Applied Microbiology and Biotechnology Biotechnology |
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