NDLI: Molecular Biological Detection of Anaerobic Gut Fungi (Neocallimastigales) from Landfill Sites

Content Provider	PubMed Central
Author	Lockhart, Robert J. Dyke, Michele I. Van Beadle, Ian R. Humphreys, Paul Mccarthy, Alan J.
Copyright Year	2006
Abstract	Oligonucleotide primers were designed for the 18S rRNA genes of members of the Neocallimastigales and used in a nested PCR protocol to amplify 787-bp fragments of DNA from landfill site samples. The specificities of the primers were confirmed by phylogenetic analysis of the environmental clone sequences, and this method can therefore now be used to investigate the ecology of the obligately anaerobic fungi. To our knowledge, this is the first demonstration of the occurrence of members of the Neocallimastigales outside the mammalian gut, and their distribution across the landfill samples examined here suggests that they are actively involved in cellulose degradation.
Related Links	http://dx.doi.org/10.1128/aem.01057-06
Ending Page	5661
Page Count	3
Starting Page	5659
File Format	PDF
ISSN	00992240
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	8
Volume Number	72
Language	English
Publisher	American Society for Microbiology
Publisher Date	2006-08-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Biotechnology Food Science Ecology Applied Microbiology and Biotechnology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

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Molecular Biological Detection of Anaerobic Gut Fungi (Neocallimastigales) from Landfill Sites