NDLI: A GFP-based System to Uncouple mRNA Transport from Translation in a Single Living NeuronV⃞

Content Provider	PubMed Central
Author	Macchi, Paolo Hemraj, Indradeo Goetze, Bernhard Grunewald, Barbara Mallardo, Massimo Kiebler, Michael A.
Editor	Lippincott-schwartz, Jennifer
Copyright Year	2003
Abstract	An inducible fluorescent system based on GFP is presented that allows for the uncoupling of dendritic mRNA transport from subsequent protein synthesis at the single cell level. The iron-responsive element (IRE) derived from ferritin mRNA in the 5′-UTR of the GFP reporter mRNA renders translation of its mRNA dependent on iron. The addition of the full-length 3′-UTR of the Ca2+/calmodulin-dependent protein kinase II alpha (CaMKIIα) after the stop codon of the GFP reading frame targets the reporter mRNA to dendrites of transfected fully polarized hippocampal neurons. As we show by time-lapse videomicroscopy, iron specifically turns on GFP reporter protein synthesis in a single transfected hippocampal neuron. We investigate whether GFP expression is affected—in addition to iron—by synaptic activity. Interestingly, synaptic activity has a clear stimulatory effect. Most importantly, however, this activity-dependent protein synthesis is critically dependent on the presence of the full-length 3′-UTR of CaMKIIα confirming that this sequence contains translational activation signals. The IRE-based system represents a new convenient tool to study local protein synthesis in mammalian cells where mRNA localization to a specific intracellular compartment occurs.
Related Links	http://dx.doi.org/10.1091/mbc.E02-08-0505
Starting Page	1570
File Format	PDF
ISSN	10591524
Journal	Molecular Biology of the Cell
Issue Number	4
Volume Number	14
Language	English
Publisher	The American Society for Cell Biology
Publisher Date	2003-04-01
Access Restriction	Open
Rights Holder	The American Society for Cell Biology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A single molecule approach to mRNA transport by a class V myosin

RNA transport from transcription to localized translation: a single molecule perspective

Visualization of mRNA translation in living cells

Probing the closed-loop model of mRNA translation in living cells

Aberrant Eukaryotic Translation Initiation Factor 4E-Dependent mRNA Transport Impedes Hematopoietic Differentiation and Contributes to Leukemogenesis

A GFP-based reporter system to monitor nonsense-mediated mRNA decay

Reconstitution of functional mRNA-protein complexes in a rabbit reticulocyte cell-free translation system.

CORSEN, a new software dedicated to microscope-based 3D distance measurements: mRNA–mitochondria distance, from single-cell to population analyses

Analysis of mutations that uncouple transport from phosphorylation in enzyme IIGlc of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system.

A GFP-based System to Uncouple mRNA Transport from Translation in a Single Living NeuronV⃞

Similar Documents

A single molecule approach to mRNA transport by a class V myosin

RNA transport from transcription to localized translation: a single molecule perspective

Visualization of mRNA translation in living cells

Probing the closed-loop model of mRNA translation in living cells

Aberrant Eukaryotic Translation Initiation Factor 4E-Dependent mRNA Transport Impedes Hematopoietic Differentiation and Contributes to Leukemogenesis

A GFP-based reporter system to monitor nonsense-mediated mRNA decay

Reconstitution of functional mRNA-protein complexes in a rabbit reticulocyte cell-free translation system.

CORSEN, a new software dedicated to microscope-based 3D distance measurements: mRNA–mitochondria distance, from single-cell to population analyses

Analysis of mutations that uncouple transport from phosphorylation in enzyme IIGlc of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system.

A GFP-based System to Uncouple mRNA Transport from Translation in a Single Living NeuronV⃞