NDLI: Digalactosyldiacylglycerol Synthesis in Chloroplasts of the Arabidopsis dgd1 Mutant1

Content Provider	PubMed Central
Author	Klaus, Dörte Härtel, Heiko Fitzpatrick, Lynda M. Froehlich, John E. Hubert, Jamie Benning, Christoph Peter, Dörmann
Copyright Year	2002
Abstract	Galactolipid biosynthesis in plants is highly complex. It involves multiple pathways giving rise to different molecular species. To assess the contribution of different routes of galactolipid synthesis and the role of molecular species for growth and photosynthesis, we initiated a genetic approach of analyzing double mutants of the digalactosyldiacylglycerol (DGDG) synthase mutant dgd1 with the acyltransferase mutant, act1, and the two desaturase mutants, fad2 and fad3. The double mutants showed different degrees of growth retardation: act1,dgd1 was most severely affected and growth of fad2,dgd1 was slightly reduced, whereas fad3,dgd1 plants were very similar to dgd1. In act1,dgd1, lipid and chlorophyll content were reduced and photosynthetic capacity was affected. Molecular analysis of galactolipid content, fatty acid composition, and positional distribution suggested that the growth deficiency is not caused by changes in galactolipid composition per se. Chloroplasts of dgd1 were capable of synthesizing monogalactosyldiacylglycerol, DGDG, and tri- and tetragalactosyldiacylglycerol. Therefore, the reduced growth of act1,dgd1 and fad2,dgd1 cannot be explained by the absence of DGDG synthase activity from chloroplasts. Molecular analysis of DGDG accumulating in the mutants during phosphate deprivation suggested that similarly to the residual DGDG of dgd1, this additional lipid is synthesized in association with chloroplast membranes through a pathway independent of the mutations, act1, dgd1, fad2, and fad3. Our data imply that the severe growth defect of act1,dgd1 is caused by a reduced metabolic flux of chloroplast lipid synthesis through the eukaryotic and prokaryotic pathway as well as by the reduction of photosynthetic capacity caused by the destabilization of photosynthetic complexes.
Related Links	http://dx.doi.org/10.1104/pp.010780
Starting Page	885
File Format	PDF
ISSN	15322548
e-ISSN	15322548
Journal	Plant Physiology
Issue Number	3
Volume Number	128
Language	English
Publisher	American Society of Plant Physiologists
Publisher Date	2002-03-01
Access Restriction	Open
Rights Holder	American Society of Plant Physiologists
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Physiology Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Synthesis of mono- and digalactosyldiacylglycerol in isolated spinach chloroplasts

Synthesis of Mono- and Digalactosyldiacylglycerol in Isolated Spinach Chloroplasts 1

Disruption of the Two Digalactosyldiacylglycerol Synthase Genes DGD1 and DGD2 in Arabidopsis Reveals the Existence of an Additional Enzyme of Galactolipid Synthesis

Disruption of the two digalactosyldiacylglycerol synthase genes DGD1 and DGD2 in Arabidopsis reveals the existence of an additional enzyme of galactolipid synthesis.

Ozone-Induced Programmed Cell Death in the Arabidopsis radical-induced cell death1 Mutant1

Preexisting Systemic Acquired Resistance Suppresses Hypersensitive Response-Associated Cell Death in Arabidopsis hrl1 Mutant1

Transformation of the Collateral Vascular Bundles into Amphivasal Vascular Bundles in an Arabidopsis Mutant1

Pollen Germinates Precociously in the Anthers of raring-to-go, an Arabidopsis Gametophytic Mutant1

Characterization of N-Glycans from Arabidopsis. Application to a Fucose-Deficient Mutant1

Digalactosyldiacylglycerol Synthesis in Chloroplasts of the Arabidopsis dgd1 Mutant1

Similar Documents

Synthesis of mono- and digalactosyldiacylglycerol in isolated spinach chloroplasts

Synthesis of Mono- and Digalactosyldiacylglycerol in Isolated Spinach Chloroplasts 1

Disruption of the Two Digalactosyldiacylglycerol Synthase Genes DGD1 and DGD2 in Arabidopsis Reveals the Existence of an Additional Enzyme of Galactolipid Synthesis

Disruption of the two digalactosyldiacylglycerol synthase genes DGD1 and DGD2 in Arabidopsis reveals the existence of an additional enzyme of galactolipid synthesis.

Ozone-Induced Programmed Cell Death in the Arabidopsis radical-induced cell death1 Mutant1

Preexisting Systemic Acquired Resistance Suppresses Hypersensitive Response-Associated Cell Death in Arabidopsis hrl1 Mutant1

Transformation of the Collateral Vascular Bundles into Amphivasal Vascular Bundles in an Arabidopsis Mutant1

Pollen Germinates Precociously in the Anthers of raring-to-go, an Arabidopsis Gametophytic Mutant1

Characterization of N-Glycans from Arabidopsis. Application to a Fucose-Deficient Mutant1

Digalactosyldiacylglycerol Synthesis in Chloroplasts of the Arabidopsis dgd1 Mutant1