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| Content Provider | PubMed Central |
|---|---|
| Author | Earl, Ashlee M. Mohundro, Michael M. Mian, I. Saira Battista, John R. |
| Copyright Year | 2002 |
| Abstract | IRS24 is a DNA damage-sensitive strain of Deinococcus radiodurans strain 302 carrying a mutation in an uncharacterized locus designated irrE. Five overlapping cosmids capable of restoring ionizing radiation resistance to IRS24 were isolated from a genomic library. The ends of each cloned insert were sequenced, and these sequences were used to localize irrE to a 970-bp region on chromosome I of D. radiodurans R1. The irrE gene corresponds to coding sequence DR0167 in the R1 genome. The irrE gene encodes a 35,000-Da protein that has no similarity to any previously characterized peptide. The irrE locus of R1 was also inactivated by transposon mutagenesis, and this strain was sensitive to ionizing radiation, UV light, and mitomycin C. Preliminary findings indicate that IrrE is a novel regulatory protein that stimulates transcription of the recA gene following exposure to ionizing radiation. |
| Related Links | http://dx.doi.org/10.1128/jb.184.22.6216-6224.2002 |
| Ending Page | 6224 |
| Page Count | 9 |
| Starting Page | 6216 |
| File Format | |
| ISSN | 00219193 |
| e-ISSN | 10985530 |
| Journal | Journal of Bacteriology |
| Issue Number | 22 |
| Volume Number | 184 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 2002-11-15 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Molecular Biology Microbiology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Microbiology |
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