NDLI: The Cf-9 Disease Resistance Protein Is Present in an ∼420-Kilodalton Heteromultimeric Membrane-Associated Complex at One Molecule per Complex

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Zein Protein Interactions, Rather Than the Asymmetric Distribution of Zein mRNAs on Endoplasmic Reticulum Membranes, Influence Protein Body Formation in Maize Endosperm

The Cf-9 Disease Resistance Protein Is Present in an ∼420-Kilodalton Heteromultimeric Membrane-Associated Complex at One Molecule per Complex

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Year: 2002, Volume: 14, Issue: 2

Year: 2002, Volume: 14, Issue: 1

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The Cf-9 Disease Resistance Protein Is Present in an ∼420-Kilodalton Heteromultimeric Membrane-Associated Complex at One Molecule per Complex

Content Provider	PubMed Central
Author	Rivas, Susana Romeis, Tina Jones, Jonathan D. G.
Copyright Year	2002
Abstract	The tomato Cf-9 gene confers race-specific resistance to the fungal pathogen Cladosporium fulvum expressing the corresponding avirulence gene Avr9. In tobacco, Cf-9 confers a hypersensitive response to the Avr9 peptide. To investigate Cf-9 protein function in initiating defense signaling, we engineered a functional C-terminal fusion of the Cf-9 gene with the TAP (Tandem Affinity Purification) tag. In addition, we established a transient expression assay in Nicotiana benthamiana leaves for the production of functional Cf-9:myc and Cf-9:TAP. Transiently expressed Cf-9:myc and Cf-9:TAP proteins induced an Avr9-dependent hypersensitive response, consistent with previous results with stably transformed tobacco plants and derived cell suspension cultures expressing c-myc–tagged Cf-9. Gel filtration of microsomal fractions solubilized with octylglucoside revealed that the Cf-9 protein, either as c-myc or TAP fusions, migrated at a molecular mass of 350 to 475 kD. By using blue native gel electrophoresis, the molecular size was confirmed to be ∼420 kD. Our results suggest that only one Cf-9 protein molecule is present in the Cf-9 complex and that Cf-9 is part of a membrane complex consisting of an additional glycoprotein partner(s). The high structural similarity between Cf proteins and Clavata2 (CLV2) of Arabidopsis, together with the similarity of molecular mass between Cf-9 and CLV complexes (420 and 450 kD, respectively), led us to investigate whether Cf-9 is integrated into membrane-associated protein complexes like those formed by CLV1 and CLV2. Unlike CLV2, the Cf-9 protein did not form disulfide-linked heterodimers, no ligand (Avr9)-dependent shift in the molecular mass of the Cf-9 complex was detected, and no Rho-GTPase–related proteins were found associated with Cf-9 under the conditions tested. Thus, Cf-9–dependent defense signaling and CLV2-dependent regulation of meristem development seem to be accomplished via distinct mechanisms, despite the structural similarity of their key components Cf-9 and CLV2.
Related Links	http://dx.doi.org/10.1105/tpc.010357
Ending Page	702
Page Count	14
Starting Page	689
File Format	PDF
ISSN	10404651
e-ISSN	1532298X
Journal	The Plant Cell
Issue Number	3
Volume Number	14
Language	English
Publisher	American Society of Plant Biologists
Publisher Date	2002-03-01
Access Restriction	Open
Rights Holder	American Society of Plant Biologists
Subject Keyword	Plant Science Cell Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science

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The Cf-9 disease resistance protein is present in an approximately 420-kilodalton heteromultimeric membrane-associated complex at one molecule per complex.

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Multidrug Resistance-associated Protein 9 (ABCC12) is present in mouse and boar sperm

The tomato Cf-9 disease resistance gene functions in tobacco and potato to confer responsiveness to the fungal avirulence gene product avr 9

Identification of Two Genes Required in Tomato for Full Cf-9-Dependent Resistance to Cladosporium fulvum.

The Cf-9 Disease Resistance Protein Is Present in an ∼420-Kilodalton Heteromultimeric Membrane-Associated Complex at One Molecule per Complex

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The Cf-9 disease resistance protein is present in an approximately 420-kilodalton heteromultimeric membrane-associated complex at one molecule per complex.

Functional Analysis of Avr9/Cf-9 Rapidly Elicited Genes Identifies a Protein Kinase, ACIK1, That Is Essential for Full Cf-9–Dependent Disease Resistance in TomatoW⃞

Purification of the 170- to 180-kilodalton membrane glycoprotein associated with multidrug resistance. 170- to 180-kilodalton membrane glycoprotein is an ATPase.

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The Cf-9 Disease Resistance Protein Is Present in an ∼420-Kilodalton Heteromultimeric Membrane-Associated Complex at One Molecule per Complex