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| Content Provider | PubMed Central |
|---|---|
| Author | Sutthent, Ruengpung Gaudart, Narintorn Chokpaibulkit, Kulkanya Tanliang, Nattaya Chinda, Kanoksinsombath Chaisilwatana, Pongsakdi |
| Copyright Year | 2003 |
| Abstract | We modified a p24 antigen enzyme-linked immunosorbent assay as a method for diagnosis and monitoring of human immunodeficiency virus type 1 (HIV-1) subtype E infection. This modified assay is based on the use of preheated immune complex dissociation combined with a booster step using a regular Vironostika HIV-1 p24 antigen assay (bioMerieux) to decrease the lower limit of p24 antigen detection from 10 pg/ml (lower limit achievable when using a regular p24 antigen assay) to 0.5 pg/ml (100 virions/ml) by the new method. The correlation between the values obtained by the HIV-1 RNA (Amplicor HIV-1 Monitor) assay and the p24 antigen assay modified with a booster step antigen assay in 160 frozen plasma samples with known viral load and 80 blind fresh plasma samples by Spearman rank were 0.671 (R 2 = 0.450; P < 0.01) and 0.782 (R 2 = 0.612; P < 0.01). During antiretroviral treatment, the change of p24 antigen level at ≥0.5 log correlated well with the level of HIV-1 in plasma. In order to improve the early diagnosis of HIV-1 infection in 121 infants born to HIV-1-infected mothers, a heat-denatured plasma p24 antigen assay modified with a booster step was compared with DNA-PCR and HIV RNA (nucleic acid sequence-based amplification) assays. The sensitivity of the antigen test modified with a booster step was similar to that of the HIV-1 RNA (NASBA QL) assay and better than that of the DNA-PCR assay (100 versus 61.90%) for subjects 1 to 2 months old. The overall results from this study might renew interest in p24 antigen detection as an easily affordable alternative method for diagnosis of HIV-1 infection and monitoring of disease progression in developing countries. |
| Related Links | http://dx.doi.org/10.1128/jcm.41.3.1016-1022.2003 |
| Ending Page | 1022 |
| Page Count | 7 |
| Starting Page | 1016 |
| File Format | |
| ISSN | 00951137 |
| e-ISSN | 1098660X |
| Journal | Journal of Clinical Microbiology |
| Issue Number | 3 |
| Volume Number | 41 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 2003-03-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Microbiology (medical) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology (medical) |
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