NDLI: Laser-Capture Microdissection, a Tool for the Global Analysis of Gene Expression in Specific Plant Cell Types

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Year: 2003, Volume: 15, Issue: 2

Year: 2003, Volume: 15, Issue: 1

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Laser-Capture Microdissection, a Tool for the Global Analysis of Gene Expression in Specific Plant Cell Types

Content Provider	PubMed Central
Author	Nakazono, Mikio Qiu, Fang Borsuk, Lisa A. Schnable, Patrick S.
Copyright Year	2003
Abstract	Laser-capture microdissection (LCM) allows for the one-step procurement of large homogeneous populations of cells from tissue sections. In mammals, LCM has been used to conduct cDNA microarray and proteomics studies on specific cell types. However, LCM has not been applied to plant cells, most likely because plant cell walls make it difficult to separate target cells from surrounding cells and because ice crystals can form in the air spaces between cells when preparing frozen sections. By fixing tissues, using a cryoprotectant before freezing, and using an adhesive-coated slide system, it was possible to capture large numbers (>10,000) of epidermal cells and vascular tissues (vascular bundles and bundle sheath cells) from ethanol:acetic acid–fixed coleoptiles of maize. RNA extracted from these cells was amplified with T7 RNA polymerase and used to hybridize a microarray containing ∼8800 maize cDNAs. Approximately 250 of these were expressed preferentially in epidermal cells or vascular tissues. These results demonstrate that the combination of LCM and microarrays makes it feasible to conduct high-resolution global gene expression analyses of plants. This approach has the potential to enhance our understanding of diverse plant cell type–specific biological processes.
Related Links	http://dx.doi.org/10.1105/tpc.008102
Ending Page	596
Page Count	14
Starting Page	583
File Format	PDF
ISSN	10404651
e-ISSN	1532298X
Journal	The Plant Cell
Issue Number	3
Volume Number	15
Language	English
Publisher	American Society of Plant Biologists
Publisher Date	2003-03-01
Access Restriction	Open
Rights Holder	American Society of Plant Biologists
Subject Keyword	Plant Science Cell Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science

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