NDLI: Adaptor and Clathrin Exchange at the Plasma Membrane and trans-Golgi Network

Content Provider	PubMed Central
Author	Wu, Xufeng Zhao, Xiaohong Puertollano, Rosa Bonifacino, Juan S. Eisenberg, Evan Greene, Lois E.
Editor	Schekman, Randy
Copyright Year	2003
Abstract	We previously demonstrated, using fluorescence recovery after photobleaching, that clathrin in clathrin-coated pits at the plasma membrane exchanges with free clathrin in the cytosol, suggesting that clathrin-coated pits are dynamic structures. We now investigated whether clathrin at the trans-Golgi network as well as the clathrin adaptors AP2 and AP1 in clathrin-coated pits at the plasma membrane and trans-Golgi network, respectively, also exchange with free proteins in the cytosol. We found that when the budding of clathrin-coated vesicle is blocked without significantly affecting the structure of clathrin-coated pits, both clathrin and AP2 at the plasma membrane and clathrin and AP1 at the trans-Golgi network exchange rapidly with free proteins in the cytosol. In contrast, when budding of clathrin-coated vesicles was blocked at the plasma membrane or trans-Golgi network by hypertonic sucrose or K+ depletion, conditions that markedly affect the structure of clathrin-coated pits, clathrin exchange was blocked but AP2 at the plasma membrane and both AP1 and the GGA1 adaptor at the trans-Golgi network continue to rapidly exchange. We conclude that clathrin-coated pits are dynamic structures with rapid exchange of both clathrin and adaptors and that adaptors are able to exchange independently of clathrin when clathrin exchange is blocked.
Related Links	http://dx.doi.org/10.1091/mbc.e02-06-0353
Ending Page	528
Page Count	13
Starting Page	516
File Format	PDF
ISSN	10591524
Journal	Molecular Biology of the Cell
Issue Number	2
Volume Number	14
Language	English
Publisher	The American Society for Cell Biology
Publisher Date	2003-02-01
Access Restriction	Open
Rights Holder	The American Society for Cell Biology
Subject Keyword	Cell Biology Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Phosphoinositide-mediated clathrin adaptor progression at the trans Golgi network

Visualization of Protein Sorting at the Trans-Golgi Network and Endosomes Through Super-Resolution Imaging

Eps15 Mediates Vesicle Trafficking from the trans-Golgi Network via an Interaction with the Clathrin Adaptor AP-1

Glucose regulates clathrin adaptors at the trans-Golgi network and endosomes

Mechanisms of Phosphoinositide-Mediated Clathrin Adaptor Progression at the trans-Golgi Network

Energy metabolism regulates clathrin adaptors at the trans-Golgi network and endosomes

Actin dynamics coupled to clathrin-coated vesicle formation at the trans-Golgi network

Clathrin-dependent Association of CVAK104 with Endosomes and the Trans-Golgi Network

The Clathrin Adaptor Gga2p Is a Phosphatidylinositol 4-phosphate Effector at the Golgi Exit

Adaptor and Clathrin Exchange at the Plasma Membrane and trans-Golgi Network

Similar Documents

Phosphoinositide-mediated clathrin adaptor progression at the trans Golgi network

Visualization of Protein Sorting at the Trans-Golgi Network and Endosomes Through Super-Resolution Imaging

Eps15 Mediates Vesicle Trafficking from the trans-Golgi Network via an Interaction with the Clathrin Adaptor AP-1

Glucose regulates clathrin adaptors at the trans-Golgi network and endosomes

Mechanisms of Phosphoinositide-Mediated Clathrin Adaptor Progression at the trans-Golgi Network

Energy metabolism regulates clathrin adaptors at the trans-Golgi network and endosomes

Actin dynamics coupled to clathrin-coated vesicle formation at the trans-Golgi network

Clathrin-dependent Association of CVAK104 with Endosomes and the Trans-Golgi Network

The Clathrin Adaptor Gga2p Is a Phosphatidylinositol 4-phosphate Effector at the Golgi Exit

Adaptor and Clathrin Exchange at the Plasma Membrane and trans-Golgi Network